Human Siglec-5/Siglec-14 MAb (Clone 194128) (25 UG)【科瑞奥博】

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Human Siglec-5/Siglec-14 MAb (Clone 194128) (25 UG)

货号： MAB10721-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB10721-SP
别名	Siglec-5/Siglec-14
全称	Sialic Acid Binding Ig-like Lectin 5
反应种属	Human
应用	Flow Cytometry,CyTOF-ready,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human Siglec‑5/Siglec‑14 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Siglec‑5-mediated adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The Neutralization Dose (ND₅₀) is typically 0.04-0.2 µg/mL in the presence of 5 µg/mL Recombinant Human Siglec‑5 Fc Chimera.
来源	Monoclonal Mouse IgG₁ Clone # 194128
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8778 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides. Authors: Izquierdo-Useros N, Lorizate M, Puertas M, Rodriguez-Plata M, Zangger N, Erikson E, Pino M, Erkizia I, Glass B, Clotet B, Keppler O, Telenti A, Krausslich H, Martinez-Picado J PLoS Biol, 2012;10(12):e1001448. Species: Human Sample Type: whole cells Application: Neut Sialic acid residues play a pivotal role in alpha(1)-acid glycoprotein (AGP)-induced generation of reactive oxygen species in chemotactic peptide pre-activated neutrophil granulocytes. Authors: Gunnarsson P, Fornander L, Pahlsson P, Grenegard M Inflamm. Res., 2010;59(2):89-95. Species: Human Sample Type: Whole Cells Application: Flow Group B Streptococcus suppression of phagocyte functions by protein-mediated engagement of human Siglec-5. Authors: Carlin AF, Chang YC, Areschoug T, Lindahl G, Hurtado-Ziola N, King CC, Varki A, Nizet V J. Exp. Med., 2009;206(8):1691-9. Species: Human Sample Type: Whole Cells Application: Neut Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates. Authors: Angata T, Hayakawa T, Yamanaka M, Varki A, Nakamura M FASEB J., 2006;20(12):1964-73. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human Siglec‑5/Siglec‑14 Lys18-Thr434 Accession # O15389
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Siglecs (sialic acid binding Ig-like lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5 binds to alpha-2, 3- and alpha-2, 6-linked sialic acid equally. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in hematopoietic tissues. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.
运输条件	Blue Ice
存放说明	4℃
参考文献	Dendritic Cell Activation Dendritic Cell ITIM/ITAM Immunoreceptors Ig-type Lectins ITIM/ITAM Immunoreceptors and Related Molecules Neutrophils

参考图片

Cell Adhesion Mediated by Siglec‑5 and Neutralization by Human Siglec‑5/14 Antibody. Recombinant Human Siglec‑5 Fc Chimera (Catalog # 1072-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec‑5 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Siglec‑5/14 Monoclonal Antibody (Catalog # MAB10721). The ND₅₀ is typically 0.04-0.2 µg/mL.

Detection of Siglec‑5/14 in Human Monocytes by Flow Cytometry. Human blood derived monocytes were stained with Mouse Anti-Human Siglec‑5/14 Monoclonal Antibody (Catalog # MAB10721, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)₂ Secondary Antibody (Catalog # F0102B).