| 货号 | MAB2259-SP |
| 别名 | cathepsin L; cathepsin L1; CATL; CTSL; CTSL1; CTSLEC 3.4.22.15; EC 3.4.22; FLJ31037; Major excreted protein; MEP |
| 应用 | Western Blot,Immunoprecipitation |
| 目标/特异性 | Detects pro form of Insect Cathepsin L in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Cathepsin S is observed and no cross-reactivity with rhCathepsin B, F, K, L2/V, and activated, mature insect Cathepsin L is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1514 (Human); 1514 (Human); 13039 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Characterization of cathepsin L secreted by Sf21 insect cells. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | S. frugiperda ovarian cell line Sf 21-derived Insect Cathepsin L |
| 生物活性 | Insect |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | The pro form of insect cell Cathepsin L is stable at neutral pH, but autoactivates in acidic buffers under reducing conditions. The activated enzyme is similar in substrate specificity to mammalian Cathepsin L, and is very active as a protease, as judged by its ability to degrade azocasein. Cystatins A, B, C, E/M, F, SA, and SN are potent inhibitors of the insect cell Cathepsin L. Like other cysteine proteases related to papain, insect cell Cathepsin L is also inhibited by E-64 and reagents which react with thiols. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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