Human TREM-1 MAb (Clone 193015) (25 UG)【科瑞奥博】

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Human TREM-1 MAb (Clone 193015) (25 UG)

货号： MAB1278-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1278-SP
简介	Clone 193015 was used by HLDA to establish CD designation
别名	CD354 antigen; CD354; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1
全称	Triggering Receptor Expressed on Myeloid Cells 1
反应种属	Human
应用	Flow Cytometry,Agonist Activity,CyTOF-ready
目标/特异性	Detects human TREM-1 in direct ELISAs.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Agonist Activity: Measured by its ability to stimulate TNF-alpha secretion by human peripheral blood mononuclear cells [Bouchon, A. et al. (2001) Nature 410:1103 and Bouchon, A. et al. (2000) J. Immunology 164:4991]. The ED₅₀ for this effect is typically 1-4 μg/mL.
来源	Monoclonal Mouse IgG₁ Clone # 193015
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	54210 (Human); 58217 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cutting Edge: identification of neutrophil PGLYRP1 as a ligand for TREM-1. Authors: Read C, Kuijper J, Hjorth S, Heipel M, Tang X, Fleetwood A, Dantzler J, Grell S, Kastrup J, Wang C, Brandt C, Hansen A, Wagtmann N, Xu W, Stennicke V J Immunol, 2015;194(4):1417-21. Species: Human Sample Type: Whole Cells Application: Flow Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker in vitro and in vivo. Authors: Bosco MC, Pierobon D, Blengio F, Raggi F, Vanni C, Gattorno M, Eva A, Novelli F, Cappello P, Giovarelli M, Varesio L Blood, 2011;117(9):2625-39. Species: Human Sample Type: Cell Lysates Application: WB Comparison of gene expression profiles between human and mouse monocyte subsets. Authors: Ingersoll MA, Spanbroek R, Lottaz C Blood, 2010;115(3):e10-9. Species: Human Sample Type: Monocytes Application: Flow Intervention of an inflammation amplifier, triggering receptor expressed on myeloid cells 1, for treatment of autoimmune arthritis. Authors: Murakami Y, Akahoshi T, Aoki N, Toyomoto M, Miyasaka N, Kohsaka H Arthritis Rheum., 2009;60(6):1615-23. Species: Human Sample Type: Whole Cells Application: Functional Assay Effects of TREM-1 activation in human neutrophils: activation of signaling pathways, recruitment into lipid rafts and association with TLR4. Authors: Fortin CF, Lesur O, Fulop T Int. Immunol., 2007;19(1):41-50. Species: Human Sample Type: Whole Cells Application: Neut TREM-1 expression in tumor-associated macrophages and clinical outcome in lung cancer. Authors: Ho CC, Liao WY, Wang CY, Lu YH, Huang HY, Chen HY, Chan WK, Chen HW, Yang PC Am. J. Respir. Crit. Care Med., 2007;177(7):763-70. Species: Human Sample Type: Whole Cells Application: Bioassay Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report. Authors: Horonenko G, Hoyt JC, Robbins RA, Singarajah CU, Umar A, Pattengill J, Hayden JM Chest, 2007;132(1):58-63. Species: Human Sample Type: BALF Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TREM-1 Ala21-Asn205 Accession # Q9NP99
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Measured by its ability to stimulate TNF-alpha secretion by human peripheral blood mononuclear cells [Bouchon, A. et al. (2001) Nature 410:1103 and Bouchon, A. et al. (2000) J. Immunology 164:4991]. The ED₅₀ for this effect is typically 1-4 μg/mL.
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).
运输条件	Blue Ice
存放说明	4℃
参考文献	Bouchon, A. (2000) J. Immunol. 164:4991. Bouchon, A. (2001) Nature 410:1103. Nathan, C. and A. Ding (2001) Nature Med. 7:530.

参考图片

Detection of TREM‑1 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human TREM‑1 Monoclonal Antibody (Catalog # MAB1278, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.