| 货号 | MAB1123-SP |
| 别名 | beta-galactosidase 2; beta-galactosidase protective protein; Carboxypeptidase C; Carboxypeptidase L; cathepsin ANGBE; CTSA; EC 3.4.16; EC 3.4.16.5; GLB2; GSL; Lysosomal Carboxypeptidase A; lysosomal protective protein; PPCA; PPGBprotective protein for beta-galactosidase (galactosialidosis); Protective protein cathepsin A; Protective protein for beta-galactosidase |
| 反应种属 | Mouse |
| 应用 | Western Blot,Immunoprecipitation |
| 目标/特异性 | Detects mouse Cathepsin A/Lysosomal Carboxypeptidase A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin A or recombinant mouse (rm) DPPIV/CD26 is observed. In Western blots, it recognizes the single chain (55 kDa) form of rmCathepsin A, which can be processed into heavy (32 kDa) and light (20 kDa) chains. It recognizes the heavy chain, but not the light chain. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5476 (Human); 19025 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin A/Lysosomal Carboxypeptidase A Ala24-Tyr474 (predicted) Accession # P16675 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1, 2). Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH. Also known as protective protein, its association with beta -galactosidase ( beta -gal) and neuraminidase is essential for beta -gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of cathepsin A causes the lysosomal storage disorder galactosialidosis, characterized by a combined secondary deficiency of beta -gal and neuraminidase. Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including tachykinins, indicating that extralysosomal cathepsin A plays a role in regulation of functions of these molecules (3). Cathepsin A is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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