| 货号 | MAB1139-SP |
| 简介 | Clone 191240 was used by HLDA to establish CD designation |
| 别名 | CD329; CDw329; FOAP-9; OBBP-Like; Protein FOAP-9; sialic acid binding Ig-like lectin 9; sialic acid-binding Ig-like lectin 9; siglec-9 | 全称 | Sialic Acid Binding Ig-like Lectin 9 |
| 反应种属 | Human |
| 应用 | Western Blot,Flow Cytometry,CyTOF-ready,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | Detects human Siglec-9 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Siglec‑E is observed and less than 1% cross-reactivity with recombinant human (rh) Siglec-3, rhSiglec-5, rhSiglec-6, rhSiglec-7, rhSiglec-8 or rhSiglec-10 is observed. In Western blots, approximately 100% cross-reactivity with recombinant mouse Siglec‑E is observed under non-reduced conditions. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/106cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Siglec‑9-mediated adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The Neutralization Dose (ND50) is typically 0.015-0.075 µg/mL in the presence of 10 µg/mL Recombinant Human Siglec‑9 Fc Chimera. |
| 来源 | Monoclonal Mouse IgG2A Clone # 191240 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 27180 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Molecular mimicry of host sialylated glycans allows a bacterial pathogen to engage neutrophil Siglec-9 and dampen the innate immune response. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Siglec-9 Gln18-Gly348 (predicted) Accession # Q9Y336 |
| 内毒素水平 | <1.0 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Siglecs(1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec-5 to -11 (1‑4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. The cDNA of human Siglec-9 encodes a 463 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5, 6). In peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T cells (5). It binds equally well to both 2,3- and 2,6-linked sialic acid (5, 6). Siglec-9 is closely related to Siglec-7, and they share ~80% amino acid sequence identity. The gene encoding siglec-9 was mapped to chromosome 19q13.4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Siglec‑9 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes werestained with (A) Mouse Anti-Human Siglec-3/CD33 PE-conjugated Monoclonal Antibody(Catalog # FAB1137P) and (B) MouseAnti-Human Siglec‑9 Monoclonal Antibody (Catalog # MAB1139, filled histogram)or isotype control antibody (Catalog # MAB003,open histogram), followed by PerCP-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0114). | |
Cell Adhesion Mediated by Siglec‑9 and Neutralization by Human Siglec‑9 Antibody. Recombinant Human Siglec‑9 Fc Chimera (Catalog # 1139-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec‑9 Fc Chimera (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Siglec‑9 Monoclonal Antibody (Catalog # MAB1139). The ND50 is typically 0.015‑0.075 µg/mL. |
