| 货号 | MAB1275-SP |
| 别名 | FIL1 delta; IL1F5; IL-1HY1; IL-1L1; IL-1RP3; IL36RA; IL-36RA; IL36RN; Interleukin 36 Receptor Antagonist; Interleukin-36 Receptor Antagonist | 全称 | Interleukin 36 Receptor Antagonist/Interleukin 1 Family 5 |
| 反应种属 | Human |
| 应用 | Western Blot |
| 目标/特异性 | Detects human IL-36Ra/IL-1F5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-1ra, rhIL-18, rhIL-1F6, or rhIL-1F8. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 26525 (Human); 54450 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human IL-36Ra/IL-1F5 Met1-Asp155 Accession # Q9UBH0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Human interleukin 1 family member #5 [IL-1F5; also named FIL-1δ (delta), IL-1HY1, IL-1H3, and IL-1L1] is a member of the IL-1 family of proteins (1‑5). IL-1 family members include IL-1 beta, IL-1 alpha, IL-1ra, IL-18, and IL-1F5 - IL-1F10 (6). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that underwent multiple duplications (6). The human IL-1F5 gene is in closest proximity to the gene for IL-1ra and is likely a relatively recent duplication of the IL-1ra gene (2, 3). IL-1F5 is synthesized as a 155 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2‑5). Nevertheless, it appears to be secreted as a 17 kDa monomer (5). There is an alternate start site that potentially gives rise to an alternate splice form (5). The translated product, however, has a premature stop codon, resulting in a truncated 16 aa peptide. Human to mouse, full length IL-1F5 has 90% aa identity. Within the family, IL-1F5 is 50% aa identical to IL-1ra, and 32%, 31%, 35%, 37%, 32%, and 42% aa identical to IL‑1 beta, IL-1F6, F7, F8, F9, and F10, respectively. Cells reported to express IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 7). The receptor for IL-1F5 has not been positively identified. Indirect evidence suggests it is IL-1 Rrp2 and/or IL‑1 RAcP (8). In either case, activity association with receptor binding is also unclear. It was initially reported to be an antagonist of IL-1F9 activity (4, 6). This would be consistent with its hypothesized relationship to IL-1ra. Studies, however, find IL-1F5 antagonist activity difficult to demonstrate (8). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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