Human/Rat Osteocalcin MAb (Clone 190125) (25 UG)【科瑞奥博】

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Human/Rat Osteocalcin MAb (Clone 190125) (25 UG)

货号： MAB1419-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1419-SP
别名	BGLAP; BGP; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate (gla) protein; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein; OC; OCN; osteocalcin
全称	Bone gamma-Carboxyglutamate [gla] Protein
反应种属	Human/Rat
应用	Immunohistochemistry,CyTOF-ready,Immunocytochemistry,Intracellular Staining by Flow Cytometry
目标/特异性	Detects human Osteocalcin in direct ELISAs.
使用方法	Immunohistochemistry: 8-25 µg/mL Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	632 (Human); 12096 (Mouse); 25295 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Transcriptome sequencing wide functional analysis of human mesenchymal stem cells in response to TLR4 ligand Sci Rep, 2016;6(0):30311. Species: Human Sample Type: Whole Cells Application: IHC - PFA fixed Ultra-Porous Nanoparticle Networks: A Biomimetic Coating Morphology for Enhanced Cellular Response and Infiltration Authors: N Nasiri, A Ceramidas, S Mukherjee, A Panneersel, DR Nisbet, A Tricoli Sci Rep, 2016;6(0):24305. Species: Mouse Sample Type: Whole Cells Application: IHC Fresh Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering Stem Cell Reports, 2016;7(1):126-37. Species: Human Sample Type: Whole Cells Application: IHC - PFA fixed Primary osteoblast-like cells from patients with end-stage kidney disease reflect gene expression, proliferation, and mineralization characteristics ex vivo. Authors: Pereira R, Delany A, Khouzam N, Bowen R, Freymiller E, Salusky I, Wesseling-Perry K Kidney Int, 2015;87(3):593-601. Species: Human Sample Type: Whole Cells Application: IHC Not Specified PDL regeneration via cell homing in delayed replantation of avulsed teeth. Authors: Zhu W, Zhang Q, Zhang Y, Cen L, Wang J J Transl Med, 2015;13(0):357. Species: Canine Sample Type: Whole Tissue Application: IHC - Paraffin embedded Identification of a cell-of-origin for fibroblasts comprising the fibrotic reticulum in idiopathic pulmonary fibrosis. Authors: Xia H, Bodempudi V, Benyumov A, Hergert P, Tank D, Herrera J, Braziunas J, Larsson O, Parker M, Rossi D, Smith K, Peterson M, Limper A, Jessurun J, Connett J, Ingbar D, Phan S, Bitterman P, Henke C Am J Pathol, 2014;184(5):1369-83. Species: Human Sample Type: Whole Cells Application: IF Bone matrix, cellularity, and structural changes in a rat model with high-turnover osteoporosis induced by combined ovariectomy and a multiple-deficient diet. Authors: Govindarajan P, Bocker W, El Khassawna T, Kampschulte M, Schlewitz G, Huerter B, Sommer U, Durselen L, Ignatius A, Bauer N, Szalay G, Wenisch S, Lips K, Schnettler R, Langheinrich A, Heiss C Am J Pathol, 2014;184(3):765-77. Species: Rat Sample Type: Whole Tissue Application: IHC Dilatational band formation in bone. Authors: Poundarik, Atharva, Diab, Tamim, Sroga, Grazyna, Ural, Ani, Boskey, Adele L, Gundberg, Caren M, Vashishth, Deepak Proc Natl Acad Sci U S A, 2012;109(47):19178-83. Species: Human Sample Type: tissue Application: IHC - Not specified Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. Authors: Asumda FZ, Chase PB BMC Cell Biol., 2011;12(0):44. Species: Rat Sample Type: Whole Cells Application: ICC The guidance of human mesenchymal stem cell differentiation in vitro by controlled modifications to the cell substrate. Authors: Curran JM, Chen R, Hunt JA Biomaterials, 2006;27(27):4783-93. Species: Human Sample Type: Whole Cells Application: ICC A hybrid coating of biomimetic apatite and osteocalcin. Authors: Krout A, Wen HB, Hippensteel E, Li P J Biomed Mater Res A, 2005;73(4):377-87. Species: Rat Sample Type: Whole Tissue Application: IHC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Human Osteocalcin synthetic peptide YLYQWLGAPVPYPDPLEPRREVCELNPDCDELADHIGFQEAYRRFYGPV Accession # P02818
生物活性	Human, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Osteocalcin, also known as Bone gamma -Carboxyglutamic Acid Protein, is a secreted protein whose expression is restricted to cells of the osteoblast lineage (1). It has been frequently used as a marker for osteoblast lineage cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Lian, J.B. et al. (1999) Vitamin. Horm. 55:443.

参考图片

Osteocalcin in MG‑63 Human Cell Line. Osteocalcin was detected in immersion fixed MG‑63 human osteosarcoma cell line using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Osteocalcin in Human Osteocytes. Osteocalcin was detected in human mesenchymal stem cells differentiated into osteocytes using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Osteocalcin in Rat Osteocytes. Osteocalcin was detected in immersion fixed rat osteocytes differentiated from mesenchymal stem cells using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Osteocalcin in Human Cartilage. Osteocalcin was detected in immersion fixed paraffin-embedded sections of human cartilage using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of chondrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Osteocalcin in Human Osteosarcoma. Osteocalcin was detected in immersion fixed paraffin-embedded sections of human osteosarcoma using Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Osteocalcin in Saos‑2 Human Cell Line by Flow Cytometry. Saos‑2 human osteosarcoma cell line was stained with Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of Osteocalcin in Human Osteoblasts by Flow Cytometry. Human osteoblasts were stained with Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.