| 货号 | MAB1585-SP |
| 别名 | EC 3.4.21; EC 3.4.21.9; Enterokinase; ENTK; ENTKenterokinase; MGC133046; protease, serine, 7 (enterokinase); PRSS7; PRSS7enteropeptidase; Serine protease 7; TMPRSS15; Transmembrane protease serine 15; transmembrane protease, serine 15 |
| 反应种属 | Human |
| 应用 | Western Blot,Immunoprecipitation |
| 目标/特异性 | Detects human Enteropeptidase/Enterokinase in direct ELISAs and Western blots. It recognizes all EK forms containing the heavy chain including the single chain precursor and the mature heterodimer consisting of a heavy and light chain. In Western blots, less than 5% cross‑reactivity with recombinant human (rh) Factor X and rhThrombin is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5651 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Intracellular co-localization of trypsin-2 and matrix metalloprotease-9: possible proteolytic cascade of trypsin-2, MMP-9 and enterokinase in carcinoma. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Enteropeptidase/Enterokinase Leu41-His1019 Accession # NP_002763 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Enterokinase (EK), also called enteropeptidase and TMPRSS15 (gene name), initiates activation of pancreatic proteases by converting trypsinogen to trypsin, which in turn activates chymotrypsin, carboxypeptidases and elastases. Located in intestinal brush border, it is a disulfide bond-linked dimer of the heavy and light chains, which are derived from the same single-chain precursor. The heavy chain consists of a short cytoplamic tail, a transmembrane, a SEA, a SRCR, a MAM, two CUB and two LDL-receptor class A domains. The light chain contains the catalytic domain of trypsin-like serine proteases. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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