| 货号 | MAB1068-SP |
| 别名 | epiregulin; ER; EREG; proepiregulin |
| 反应种属 | Mouse |
| 应用 | ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | Detects mouse Epiregulin in ELISAs. In direct ELISAs, 100% cross‑reactivity with recombinant human Epiregulin is observed, but no cross‑reactivity with recombinant mouse (rm) Epigen, recombinant human (rh) TGF-alpha, rhAmphiregulin, rmBetacellulin, rhEGF, rhHB-EGF, rhHRG-alpha, or rhHRG-beta is observed. In sandwich immunoassays, no cross‑reactivity with rmAmphiregulin, rhNRG-1-beta 1, rmBetacellulin, rmEGF, rhHeregulin, rhHB-EGF, or rhTGF‑ alpha is observed. |
| 使用方法 | ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Epiregulin-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 3 ng/mL Recombinant Mouse Epiregulin. |
| 来源 | Monoclonal Rat IgG2A Clone # 189611 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2069 (Human); 13874 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Temporal expression of growth factors triggered by epiregulin regulates inflammation development. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse Epiregulin Val56-Leu101 Accession # Q61521 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. They are all synthesized as transmembrane precursors and converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The mouse Epiregulin cDNA encodes for a 162 amino acid (aa) transmembrane precursor, with the mature soluble form comprising aa 56‑101 (2). The mode of action of Epiregulin is similar to other EGF family members in that it binds to and activates the tyrosine-kinase, ErbB-family receptors (ErbB1 through B4) (3). Although it stimulates phosphorylation of all four receptors, it appears to interact primarily with ErbB1 and ErbB4. Epiregulin has the broadest specificity of the EGF-like ligands but seems to preferentially activate heterodimeric receptor complexes (4). Epiregulin exhibits a variety of biological effects. It was originally shown to both inhibit growth of several epithelial tumor cells and stimulate growth of fibroblasts and other types of cells (1). Epiregulin expression is upregulated in a number of carcinoma cell lines. It has also been shown to be an autocrine growth factor in human epidermal keratinocytes (5). Epiregulin has also been shown to play a role in the early steps of pregnancy, regulating attachment of the blastocyst to the uterine epithelium during the implantation process (6). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by Epiregulin and Neutralization by Mouse Epiregulin Antibody. Recombinant Mouse Epiregulin (Catalog # 1068-EP) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Epiregulin (3 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti‑Mouse Epiregulin Monoclonal Antibody (Catalog # MAB1068). The ND50 is typically 0.2‑1 µg/mL. |
