| 货号 | MAB1455-SP |
| 别名 | ALB; albumin; cell growth inhibiting protein 42; DKFZp779N1935; growth-inhibiting protein 20; PRO0883; PRO0903; PRO1341; serum albumin |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot,Simple Western,CyTOF-ready,Immunocytochemistry,Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human Serum Albumin in direct ELISAs. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 213 (Human); 11657 (Mouse); 24186 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human Serum Albumin |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Albumin is the most abundant plasma protein, constituting approximately 60% of the total protein in blood. It is produced primarily by the liver and is important for the maintenance of colloidal osmotic blood pressure. Therefore, it maintains the balance of fluid between the vascular compartment and extravascular tissue. It also acts as a transport protein, carrying fatty acids, bilirubin, and various drugs and hormones. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Albumin by Western Blot. Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Albumin in Hepatocytes Derived from Human Embryonic Stem Cells. Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog # AF7094) and NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Albumin in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse Serum Albumin Monoclonal Antibody (Catalog # MAB1455, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. | |
Detection of Human Albumin by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
