Cotton Rat GRO MAb (Clone 186413) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Cotton Rat GRO MAb (Clone 186413) (25 UG)

货号： MAB1114-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1114-SP
别名	CXCL1/2/3/GRO
反应种属	Cotton Rat
应用	Western Blot,Neutralization
目标/特异性	Detects cotton rat CXCL1/2/3/GRO in direct ELISAs and Western blots. In direct ELISAs, this antibody shows 5-20% cross-reactivity with recombinant rat CXCL1 and recombinant human CXCL2 and no cross-reactivity with recombinant cottonrat CXCL10, recombinant human CXCL1, 3, 4, 5, 6, 7, 8, 9, 10, 11,12/SDF-1 alpha, rh12/SDF-1 beta, 13, 16, recombinant mouse CXCL1, 2, 4, 6, 7, 9, 10, 11,12/SDF-1 alpha, 13, 15, 16, 17,recombinant rat (rr) CXCL2, rrCXCL3/CINC-2 alpha, rr3/CINC-2 beta, or recombinant porcine CXCL8.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CXCL1/2/3/GRO-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.08-0.4 µg/mL in the presence of 5 ng/mL Recombinant Cotton Rat CXCL1/2/3/GRO.
来源	Monoclonal Mouse IgG₁ Clone # 186413
产品组分

性能
供应商	R&D Systems
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant cotton rat GRO Ala28-Lys100 Accession # AAL16934
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Cotton Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Cotton rat growth-regulated protein (GRO) is a member of the ELR⁺ CXC subfamily of chemokines (1). The cotton rat GRO cDNA encodes a 100 amino acid (aa) precursor protein with a 27 aa putative signal peptide and a 73 aa mature protein (2). Among the three human GRO proteins, mature cotton rat GRO is most closely related to CXCL1/GRO alpha, sharing approximately 77% aa sequence identity (3). It also shares 75% and 70% aa sequence identity with mature human CXCL2/GRO beta and CXCL3/GRO gamma, respectively (4). Compared with rat CXC chemokines, mature cotton rat shows 92%, 76%, and 76% aa sequence homology with rat KC/GRO/CINC-1, CINC-2, and CINC-3, respectively (5). Mature cotton rat CXCL1 also shares 74% sequence homology with mature cotton rat MIP-2 (6). Similarly to GRO proteins from other species, cotton rat CXCL1 is expected to be a potent neutrophil chemoattractant and activator. Cotton rat CXCL1 has been shown to bind and activate both mouse chemokine receptors CXCR1 and CXCR2 (7). Similarly to human CXCL1, the cotton rat CXCL1 concentration required to activate CXCR1 is at least an order of magnitude higher than that required to activate CXCR2 (7, 8). Based on studies on rodents, cotton rat CXCL1 is predicted to be produced by a wide variety of cell types including macrophages, endothelial cells, fibroblasts, astrocytes, neutrophils, keratinocytes, and intestinal epithelium (1, 9).
运输条件	Blue Ice
存放说明	4℃
参考文献	Rollins, B.J. (1997) Blood 90:909. Blanco, J.C. et al. (2001) GenBank Accession #: AAL16934. Richmond, A. et al. (1988) EMBO J. 7:2025. Haskill, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7732. Nakagawa, H. et al. (1994) Biochem. J. 301:545. Blanco, J.C. et al. (2001) GenBank Accession #: AAL26705. R&D Systems, unpublished observations. Schumacher, C. et al. (1992) Proc. Natl. Acad. Sci. USA 89:10542. Zlotnik, A.O. (2000) Immunity 12:121.

参考图片

Chemotaxis Induced by CXCL1/2/3/GRO and Neutralization by Cotton Rat CXCL1/2/3/GRO Antibody. Recombinant Cotton Rat CXCL1/2/3/GRO (Catalog # 1114-GR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CXCL1/2/3/GRO (5 ng/mL) is neutralized (green line) by increasing concentrations of Cotton Rat CXCL1/2/3/GRO Monoclonal Antibody (Catalog # MAB1114). The ND₅₀ is typically 0.08-0.4 µg/mL.