Human/Mouse/Rat p53 MAb (Clone 184721) (25 UG)【科瑞奥博】

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Human/Mouse/Rat p53 MAb (Clone 184721) (25 UG)

货号： MAB1355-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1355-SP
别名	Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53
反应种属	Human/Mouse/Rat
应用	Western Blot,Immunoprecipitation,Immunocytochemistry
目标/特异性	Detects human, mouse, and rat p53.
使用方法	Western Blot: 1 µg/mL Immunoprecipitation: 1-2 µg/500 µg cell lysate Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7157 (Human); 22059 (Mouse); 24842 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Long-term culture and expansion of primary human hepatocytes. Authors: Levy G, Bomze D, Heinz S, Ramachandran S, Noerenberg A, Cohen M, Shibolet O, Sklan E, Braspenning J, Nahmias Y Nat Biotechnol, 2015;33(12):1264-1271. Species: Human Sample Type: Cell Lysates Application: WB DISC-mediated activation of caspase-2 in DNA damage-induced apoptosis. Authors: Olsson M, Vakifahmetoglu H, Abruzzo PM, Hogstrand K, Grandien A, Zhivotovsky B Oncogene, 2009;28(18):1949-59. Species: Human Sample Type: Cell Lysates Application: WB Functional connection between p53 and caspase-2 is essential for apoptosis induced by DNA damage. Authors: Vakifahmetoglu H, Olsson M, Orrenius S, Zhivotovsky B Oncogene, 2006;25(41):5683-92. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human p53 Asp7-Asp393 Accession # P04637
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.
运输条件	Blue Ice
存放说明	4℃
参考文献	Apoptosis Transcription Factors and Regulators Cancer Biomarkers Cancer Stem Cell Transcription Factors Cell Cycle Checkpoint DNA Damage and Repair Epithelial Stem Cell Transcription Factors Hematopoietic Stem Cell Transcription Factors and Regulators ICC / IHC Images: p53 Immunoglobulin-Like Domain Transcription Factors Mesenchymal Stem Cell Transcription Factors Mini-review: DNA Damage Response Mini-review: Genomic Instability Syndromes Neural Stem Cell Transcription Factors p53 Pathway p53: More than a Transcription Factor? Parkinsons Disease Related Molecules Pluripotent Stem Cell Transcription Factors TNF Superfamily Regulators Transcription Factors and Regulators Transcription Factors in the Akt Pathway

参考图片

Detection of Human p53 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line mock-treated (-) or treated (+) with 1 μM camptothecin (CPT) for 1 hour. Human p53 was immunoprecipitated using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355). PVDF membrane was probed with HRP-conjugated Anti-Human/Mouse/Rat Polyclonal Antibody (Catalog # HAF1355). A specific band for p53 was detected at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

p53 in HeLa Human Cell Line. p53 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat p53 Monoclonal Antibody (Catalog # MAB1355) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.