| 货号 | MAB746-SP |
| 别名 | EBAFTGF-beta-4; endometrial bleeding associated factor; Endometrial bleeding-associated factor; Lefta; LEFTAProtein lefty-A; left-right determination factor 2; Left-right determination factor A; LEFTY2; LEFTYATransforming growth factor beta-4; MGC46222; Protein lefty-2; TGFB4endometrial bleeding associated factor (left-right determination, factor A;transforming growth factor beta superfamily) |
| 反应种属 | Human |
| 应用 | Western Blot |
| 目标/特异性 | Detects the long isoform of recombinant human (rh) Lefty-A (aa 78‑366) in direct ELISAs and Western blots. Does not cross-react with the short form of recombinant human Lefty-A (aa 136‑366) or with rhART, recombinant mouse (rm) Artemin, recombinant drosophila DPP, rhGDNF, rhLAP, recombinant rat MIS, rhNeurturin, rhTGF-alpha, rhTGF-beta 1, rhTGF-beta 1.2, or rmLefty-1. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7044 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Lefty-A Phe78-Pro366 Accession # O00292.2 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Lefty was first identified in a screen for undifferentiated cell-specific cDNAs from the P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned the name “Lefty”. The human orthologue was initially identified as Ebaf, Endometrial Bleeding-Associated Factor. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A and Lefty-B). By amino acid sequence, human Lefty-A and -B are more similar to each other (96%) than to either Lefty-1 or -2 in the mouse (81‑82% identical). Lefty contains the six cysteine residues that are conserved among TGF-beta related proteins and that are necessary to form the cysteine-knot structure. However, Lefty is distinct from other family members in that it has two RXXR cleavage sites, a longer carboxy terminal sequence, and it lacks the cysteine residue required for intermolecular disulfide linkage. Thus, mature forms of Lefty are larger than mature forms of other TGF‑ beta ‑related proteins. Lefty homologues have been identified in other vertebrate organisms including chick, frog, and zebrafish. Although the amino acid sequence identity is not well conserved among vertebrate species, the expression pattern of Lefty on the left side is well conserved. Furthermore its function in patterning left-right asymmetry of the developing organ systems such as the heart and lung is consistent in all vertebrate species examined. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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