Human/Mouse Glucagon MAb (Clone 181402) (25 UG)【科瑞奥博】

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Human/Mouse Glucagon MAb (Clone 181402) (25 UG)

货号： MAB1249-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1249-SP
别名	GCG; glicentin-related polypeptide; glucagon; GRPP
反应种属	Human/Mouse
应用	CyTOF-ready,Immunocytochemistry,Intracellular Staining by Flow Cytometry
目标/特异性	Detects human and mouse Glucagon.
使用方法	Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2641 (Human); 14526 (Mouse); 24952 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate. Authors: Gomez D, ODriscoll M, Sheets T, Hruban R, Oberholzer J, McGarrigle J, Shamblott M PLoS ONE, 2015;10(8):e0133862. Species: Human Sample Type: Whole Tissue Application: IHC OCT-embedded Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice. Authors: Kornete, Mara, Beauchemin, Hugues, Polychronakos, Constant, Piccirillo, Ciriaco Autoimmunity, 2013;46(4):259-68. Species: Mouse Sample Type: Whole Cells Application: Flow Stem cells derived from human fetal membranes display multilineage differentiation potential. Authors: Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U Biol. Reprod., 2007;77(3):577-88. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Human Glucagon synthetic peptide
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Glucagon is a 29 amino acid residue pancreatic peptide hormone. Itstimulates gluconeogenesis and glycogenolysis to counter theglucose-lowering action of insulin.
运输条件	Blue Ice
存放说明	4℃
参考文献	Diabetes Glucose Homeostasis ICC / IHC Images: Glucagon Metabolism Neurotransmitters, Neuroactive Molecules and Associated Enzymes Pancreatic Endoderm Pancreatic Endoderm Cell Markers Protein/Peptide Hormones and Regulators

参考图片

Glucagon in beta TC-6 Mouse Cell Line. Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Glucagon in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC‑6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.