Human E-Cadherin MAb (Clone 180215) (25 UG)【科瑞奥博】

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Human E-Cadherin MAb (Clone 180215) (25 UG)

货号： MAB1838-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1838-SP
别名	Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; E-cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin
反应种属	Human
应用	Western Blot,Simple Western,Immunohistochemistry
目标/特异性	Detects human E-Cadherin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) Cadherin-8, rhCadherin-17, recombinant mouse E-Cadherin, rhN-Cadherin, rhP-Cadherin, or rhVE‑Cadherin is observed.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Immunohistochemistry: 2-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	999 (Human); 12550 (Mouse); 83502 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Rose Bengal acetate photodynamic therapy (RBAc-PDT) induces exposure and release of Damage-Associated Molecular Patterns (DAMPs) in human HeLa cells. Authors: Panzarini, Elisa, Inguscio, Valentin, Fimia, Gian Mar, Dini, Luciana PLoS ONE, 2014;9(8):e105778. Species: Human Sample Type: Subcellular Fractions Application: WB Progression of oral squamous cell carcinoma accompanied with reduced e-cadherin expression but not cadherin switch. Authors: Hashimoto T, Soeno Y, Maeda G, Taya Y, Aoba T, Nasu M, Kawashiri S, Imai K PLoS ONE, 2012;7(10):e47899. Species: Mouse Sample Type: Whole Cells Application: ICC Glycogene expression alterations associated with pancreatic cancer epithelial-mesenchymal transition in complementary model systems. Authors: Maupin KA, Sinha A, Eugster E, Miller J, Ross J, Paulino V, Keshamouni VG, Tran N, Berens M, Webb C, Haab BB PLoS ONE, 2010;5(9):e13002. Species: Human Sample Type: Cell Lysates Application: WB Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Authors: Franciszkiewicz K, Le Floch A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Cancer Res., 2009;69(15):6249-55. Species: Human Sample Type: Whole Cells Application: Flow Vascular endothelial growth factor-A stimulates Snail expression in breast tumor cells: implications for tumor progression. Authors: Wanami LS, Chen HY, Peiro S, Garcia de Herreros A, Bachelder RE Exp. Cell Res., 2008;314(13):2448-53. Species: Human Sample Type: Whole Cells Application: WB Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity. Authors: Miyazawa J, Mitoro A, Kawashiri S, Chada KK, Imai K Cancer Res., 2004;64(6):2024-9. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human E-Cadherin Asp155-Ile707 Accession # P12830
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, andL-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E-Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each.
运输条件	Blue Ice
存放说明	4℃
参考文献	Bussemakers, M.J.G.et al. (1993) Mol. Biol. Reports 17:123. Overduin, M.et al. (1995) Science 267:386. Takeichi, M. (1991) Science 251:1451.

参考图片

Detection of Human E‑Cadherin by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB1838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E‑Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

E-Cadherin in Human Colon. E-Cadherin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB1838) at 2 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human E‑Cadherin by Simple Western^TM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for E‑Cadherin at approximately 166 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB1838). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.