Mouse Sca-1 MAb (Clone 177228) (25 UG)【科瑞奥博】

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Mouse Sca-1 MAb (Clone 177228) (25 UG)

货号： MAB1226-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1226-SP
别名	Ly6
全称	Stem Cell Antigen-1/Lymphocyte Antigen 6
反应种属	Mouse
应用	Flow Cytometry,CyTOF-ready,Immunocytochemistry
目标/特异性	Detects mouse Sca‑1/Ly6 in direct ELISAs.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	110454 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tissue-engineered vascular grafts form neovessels that arise from regeneration of the adjacent blood vessel. Authors: Hibino N, Villalona G, Pietris N FASEB J., 2011;25(8):2731-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded BCL6 is critical for the development of a diverse primary B cell repertoire. Authors: Duy C, Yu JJ, Nahar R, Swaminathan S, Kweon SM, Polo JM, Valls E, Klemm L, Shojaee S, Cerchietti L, Schuh W, Jack HM, Hurtz C, Ramezani-Rad P, Herzog S, Jumaa H, Koeffler HP, de Alboran IM, Melnick AM, Ye BH, Muschen M J. Exp. Med., 2010;207(6):1209-21. Species: Mouse Sample Type: Whole Cells Application: Flow Magnetic surface-enhanced Raman spectroscopic (M-SERS) dots for the identification of bronchioalveolar stem cells in normal and lung cancer mice. Authors: Noh MS, Jun BH, Kim S, Kang H, Woo MA, Minai-Tehrani A, Kim JE, Kim J, Park J, Lim HT, Park SC, Hyeon T, Kim YK, Jeong DH, Lee YS, Cho MH Biomaterials, 2009;30(23):3915-25. Species: Mouse Sample Type: Whole Tissue Application: IHC Intranasal HGF administration ameliorates the physiologic and morphologic changes in lung emphysema. Authors: Hegab AE, Kubo H, Yamaya M, Asada M, He M, Fujino N, Mizuno S, Nakamura T Mol. Ther., 2008;16(8):1417-26. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse Sca‑1/Ly6 C-terminally truncated Ly-6E allele Leu27-Gly119 Accession # CAA28351
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Stem Cell Antigen-1 (Sca-1) is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).
运输条件	Blue Ice
存放说明	4℃
参考文献	Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634. Spangrude, G.I. et al. (1988) Science 241:58. Spangrude, G.I. et al. (1993) Blood 82:3327. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011. Yamamoto, Y. et al. (1996, Blood 88:445. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908. Welm, B.E. et al. (2002) Dev. Biol. 245:42. Codias, E.K. et al. (1990) J. Immunol. 144:2197. Malek, T.R. et al. (1986) J. Exp. Med. 164:709. Codias, E.K. et al. (1990) J. Immunol. 145:1407. Flood, P.M. et al. (1990) J. Exp. Med. 172:115.

参考图片

Detection of Sca‑1/Ly6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE‑conjugated Monoclonal Antibody (Catalog # FAB1356P) and either (A) Rat Anti-Mouse Sca‑1/Ly6 Monoclonal Antibody (Catalog # MAB1226) or (B) Rat IgG_2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Sca‑1/Ly6 in Mouse Splenocytes. Sca‑1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse Sca‑1/Ly6 Monoclonal Antibody (Catalog # MAB1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.