| 货号 | MAB928-SP |
| 别名 | angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC 2.7.10.1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; TIE-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1 | 全称 | Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2 |
| 反应种属 | Zebrafish |
| 应用 | Western Blot,Blockade of Receptor-ligand Interaction |
| 目标/特异性 | Detects zebrafish Tie-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with human or mouse Tie‑1 and Tie‑2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.03-0.1 µg/mL of this antibody will block 50% of the binding of 25 ng/mL of Recombinant Human Angiopoietin-2 (Catalog # 623-AN) to immobilized Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928‑T2) coated at 4 µg/mL (100 µL/well). At 0.5 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Monoclonal Mouse IgG1 Clone # 176222 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7010 (Human); 21687 (Mouse); 30747 (Zebrafish) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A crucial role of caldesmon in vascular development in vivo. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant zebrafish Tie-2 Val22-His741 Accession # O73791 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Zebrafish |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Zebrafish Tie-2 cDNA encodes a 1116 amino acid (aa) residue precursor protein shares 38% sequence homology with human Tie-2 in the extracellular domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind Tie-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Angiopoietin-2 binding to Tie-2 blocked by Zebrafish Tie-2 Antibody. In a functional ELISA, Recombinant Human Angiopoietin-2 (Catalog # 623-AN) binds to immobilized Recombinant Zebrafish Tie-2 Fc Chimera (Catalog # 928‑T2) coated at 4 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Mouse Anti-Zebrafish Tie-2 Monoclonal Antibody (Catalog # MAB928). At 0.5 μg/mL, this antibody will block 90% of the binding. |
