Human IL-6 R alpha MAb (Clone 17506) (25 UG)【科瑞奥博】

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Human IL-6 R alpha MAb (Clone 17506) (25 UG)

货号： MAB227-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB227-SP
别名	CD126; IL6R
全称	Interleukin 6 Receptor alpha
反应种属	Human
应用	Western Blot,Flow Cytometry,CyTOF-ready,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human IL-6 R in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) IL-1 alpha, recombinant mouse (rm) IL-1 alpha, rhIL-1 beta, rmIL-1 beta, rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑6 R alpha -mediated enhancement of IL‑6-dependent Inhibition of cell proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND₅₀) is typically 0.04-0.2 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑6 R alpha and 30 ng/mL Recombinant Human IL‑6.
来源	Monoclonal Mouse IgG₁ Clone # 17506
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3570 (Human); 16194 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium PLoS ONE, 2016;11(5):e0155161. Species: Human Sample Type: Whole Cells Application: Neut Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells. Authors: Sommer A, Fabri M PLoS ONE, 2015;10(6):e0130395. Species: Human Sample Type: Whole Cells Application: Neut Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Nature, 2012;484(7395):514-8. Species: Human Sample Type: Whole Cells Application: Neut Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth. Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E Nat. Med., 2011;17(11):1498-503. Species: Human Sample Type: In Vivo Application: In Vivo Homing Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells. Authors: Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA Cancer Res., 2009;69(1):329-37. Species: Human Sample Type: Whole Cells Application: Flow Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells. Authors: Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F Nat. Immunol., 2009;10(8):857-63. Species: Human Sample Type: Whole Cells Application: Neut IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation. Authors: Kolliputi N, Waxman AB Am. J. Respir. Cell Mol. Biol., 2009;40(3):314-24. Species: Human Sample Type: Cell Lysates Application: WB Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F Nat. Immunol., 2007;8(9):942-9. Species: Human Sample Type: Whole Cells Application: Neut Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle. Authors: Ammit AJ, Moir LM, Oliver BG, Hughes JM, Alkhouri H, Ge Q, Burgess JK, Black JL, Roth M Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(1):L199-206. Species: Human Sample Type: Cell Lysates Application: WB trans fatty acids and systemic inflammation in heart failure. Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC Am. J. Clin. Nutr., 2004;80(6):1521-5. Species: Human Sample Type: Plasma Application: ELISA Development Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA J. Immunol., 2002;169(9):5244-51. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells. Authors: Erices A, Conget P, Rojas C, Minguell JJ Exp. Cell Res., 2002;280(1):24-32. Species: Human Sample Type: Whole Cells Application: Flow Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation. Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA Immunity, 2001;14(6):705-14. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Mechanisms of growth control of Kaposi's sarcoma-associated herpes virus-associated primary effusion lymphoma cells. Authors: Asou H, Said JW, Yang R, Munker R, Park DJ, Kamada N, Koeffler HP Blood, 1998;91(7):2475-81. Species: Human Sample Type: Cell Culture Supernates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑6 R Leu20-Asp339 Accession # P08887
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4). A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).
运输条件	Blue Ice
存放说明	4℃
参考文献	Yamasakiet al. (1988) Science 241:825. Baumannet al. (1990) J. Biol. Chem. 265:19853. Hibiet al. (1990) Cell 63:1149. Schooltinket al. (1991) Eur. J. Biochem. 277:659. Novicket al. (1989) J. Exp. Med. 170:1409. Yasukawaet al. (1990) J. Biochem. 108:673. Saitoet al. (1991) J. Immunology 147:168.

参考图片

IL‑6 R alpha Enhancement of
IL‑6-dependent Inhibition of Cell Proliferation and Neutralization by Human
IL‑6 R alpha Antibody. Recombinant Human IL‑6 R alpha (Catalog #
227-SR) enhances Recombinant Human IL‑6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL‑6 (30 ng/mL) activity elicited by Recombinant Human IL‑6 R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB227). The ND₅₀ is typically
0.04-0.2 µg/mL.

Detection of IL‑6 R alpha in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was labeled with Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG
F(ab)₂ Secondary Antibody (Catalog # F0102B).