| 货号 | MAB922-SP |
| 别名 | EDAA2; EDA-A2/Ectodysplasin A2 |
| 反应种属 | Human |
| 应用 | Western Blot |
| 目标/特异性 | Detects human EDA‑A2/Ectodysplasin A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant human (rh) EDA-A1 is observed, approximately 10% cross‑reactivity with recombinant mouse EDA-A1 is observed, and no cross‑reactivity with rhAPRIL, rhBAFF, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhOX40 Ligand, rhTNF-alpha, rhTRAIL, rhTRANCE, rhTWEAK, and rhVEGI is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1896 (Human); 13607 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human EDA‑A2/Ectodysplasin A2 Ala179-Ser389 Accession # NP_001005609 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | EDA-A2, a product of the EDA gene (also called Tabby), is a type II transmembrane protein that is a member of the TNF Superfamily (TNFSF). Human EDA-A2 is a 389 amino acid (aa) protein with a predicted N-terminal 39 aa cytoplasmic domain, a 22 aa transmembrane domain and a C-terminal 328 aa extracellular domain. The extracellular domains of human and mouse EDA-A2 share approximately 94% identity. Within the TNFSF, EDA-A2 shares the highest homology with EDA-A1, the other product of the EDA gene. EDA-A2 and EDA-A1 are splice variants of EDA which differ by only two amino acids. EDA-A1 contains two additional amino acids, Glu308 and Val309. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, a member of the TNF Receptor Superfamily (TNFRSF), whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF family member with high homology to EDAR. Mutations in EDA-A1, EDA-A2, EDAR and XEDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|