Human/Mouse Sonic Hedgehog/Shh N-Terminus MAb (Clone 171001) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human/Mouse Sonic Hedgehog/Shh N-Terminus MAb (Clone 171001) (25 UG)

货号： MAB464-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB464-SP
别名	HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; sonic hedgehog; TPT; TPTPS
反应种属	Human/Mouse
应用	Western Blot
目标/特异性	Detects the N-terminal peptide of human (amino acids 24-197) and mouse (amino acids 25-198) Shh in direct ELISAs and Western blots. Shows 50-100% cross-reactivity with the N-terminal peptides of recombinant mouse (rm) Dhh (amino acids 23‑198) and rmIhh (amino acids 66‑240) and no cross-reactivity with the C‑terminal peptides from rmShh (amino acids 199‑437), rmDhh (amino acids 199‑396), or rmIhh (amino acids 241‑449).
使用方法	Western Blot: 2 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6469 (Human); 20423 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Neuroprotective effect of sonic hedgehog up-regulated in Schwann cells following sciatic nerve injury. Authors: Hashimoto M, Ishii K, Nakamura Y, Watabe K, Kohsaka S, Akazawa C J. Neurochem., 2008;107(4):918-27. Species: Rat Sample Type: Tissue Homogenates Application: WB Wntless, a conserved membrane protein dedicated to the secretion of Wnt proteins from signaling cells. Authors: Banziger C, Soldini D, Schutt C, Zipperlen P, Hausmann G, Basler K Cell, 2006;125(3):509-22. Species: Mouse Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse Sonic Hedgehog Cys25-Gly198 (Lys122Arg) Accession # Q62226
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).
运输条件	Blue Ice
存放说明	4℃
参考文献	A Role for Sonic Hedgehog in Axon Guidance Axon Guidance and Neuron Migration EMT Induction Hedgehog Family Hedgehogs ICC / IHC Images: Sonic Hedgehog/Shh Lipid-modified Sonic Hedgehog (Shh) Mini-review: Sonic Hedgehog (Shh) Morphogens Retinal Stem Cell Markers Sonic Hedgehog: a morphogen involved in axon guidance

参考图片

Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB464) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.