Human CXCL4/PF4 MAb (Clone 170138) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human CXCL4/PF4 MAb (Clone 170138) (25 UG)

货号： MAB7952-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB7952-SP
别名	chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4iroplact; Iroplact; MGC138298; Oncostatin-A; PF4; PF-4; platelet factor 4; SCYB4oncostatin-A
反应种属	Human
应用	Western Blot,CyTOF-ready,Intracellular Staining by Flow Cytometry
目标/特异性	Detects human CXCL4/PF4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) CXCL6, recombinant human (rh) CXCL6, rhCXCL7, rmCXCL13 or rhCCL21 is observed.
使用方法	Western Blot: 0.5 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5196 (Human); 56744 (Mouse); 360918 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Functional divergence between 2 chemokines is conferred by single amino acid change. Authors: Dubrac A, Quemener C, Lacazette E, Lopez F, Zanibellato C, Wu WG, Bikfalvi A, Prats H Blood, 2010;116(22):4703-11. Species: Human Sample Type: recombinant CXCL4 Application: Binding Assay
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human CXCL4/PF4 Glu32-Ser101 Accession # P02776.2
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCL4, also known as Platelet Factor 4 (PF4), is an 8 kDa CXC chemokine that is stored in platelet alpha -granules as a homotetramer and secreted abundantly during platelet activation. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein that includes granule targeting and heparin-binding sequences. CXCL4 has homology with IL-8 and beta -thromboglobulin and can form heteromultimers with IL-8. Mature human and mouse CXCL4 share 76% aa identity. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, has anti-angiogenic activity and promotes granule Protein C activation. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.
运输条件	Blue Ice
存放说明	4℃
参考文献	Poncz, M. et al. (1987) Blood 69:219. Scheuerer, B. et al. (2000) Blood 95:1158. Perollet, C. et al. (1998) Blood 91:3289. Petersen, F. et al. (1998) J. Immunol. 161:4347. Petersen, F. et al. (1999) J. Biol. Chem. 274:12376. Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.

参考图片

Detection of Human CXCL4/PF4 by Western Blot. Western blot shows lysates of human spleen tissue and human platelets. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CXCL4/PF4 Monoclonal Antibody (Catalog # MAB7952) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.