| 货号 | MAB1128-SP |
| 别名 | BDA2; BMP-2; BMP2A; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2 | 全称 | Bone Morphogenetic Protein 2/4 |
| 反应种属 | Zebrafish |
| 应用 | Western Blot |
| 目标/特异性 | Detects zebrafish BMP‑2 and BMP‑4 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 650 (Human); 12156 (Mouse); 29373 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant zebrafish BMP‑4 Ser288-Arg400 Accession # NP_571417 |
| 生物活性 | Zebrafish |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | BMP-4 is one of at least 15 structurally and functionally-related BMPs, which are members of the transforming growth factor-beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to have roles in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta superfamily proteins, BMPs are highly conserved across animal species. Zebrafish BMP-4 shares 73% amino acid (aa) sequence identity with human and murine BMP-4, and 86% aa identity with zebrafish BMP-2b. Zebrafish BMP-4 mRNA is detected throughout embryonic development and has been detected in tissues of adult fish, including the brain, heart, digestive tracts, testes, and jaw. BMP-4 has been shown to play roles in directing zebrafish heart looping and in ear development. The combined expression pattern of zBMP-2a/2b/4 coincides with areas where BMP-2/4 expression would be found in other vertebrates. Biologically active BMP-4 is a disulfide-linked homodimer of the carboxy-terminal 113 aa that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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