| 货号 | MAB1022-SP |
| 别名 | B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; Butyrophilin-like Protein; CD273 antigen; CD273; CD273PD-1 ligand 2; MGC142240; PD-1-ligand 2; PDCD1L2MGC142238; PDCD1LG2; PDL2B7DC; PD-L2PDCD1 ligand 2; programmed cell death 1 ligand 2; Programmed death ligand 2 | 全称 | Programmed Death-1 Ligand-2 |
| 反应种属 | Mouse |
| 应用 | Western Blot,Flow Cytometry,CyTOF-ready |
| 目标/特异性 | Detects mouse PD-L2/B7-DC in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human (rh) PD-L2 is observed and no cross-reactivity with rhB7-1, recombinant mouse (rm) B7-1, rmB7-2, rmB7-H1, rmB7-H2, rmB7‑H3, or rmB7-H4 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/106cells |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 80380 (Human); 58205 (Mouse); 102146227 (Cynomolgus Monkey) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse PD-L2/B7-DC Met1-Arg219 Accession # Q9WUL5 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | PD-L2 (Programmed Death Ligand 2), also known as B7-DC and butyrophilin-like protein, is a transmembrane protein expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells. At inflammatory sites, PD-L2 is upregulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells. PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand upregulation while inhibiting IL-4 production. In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance. In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of PD‑L2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line were treated for 48 hours with 1 μg/mL LPS then stained with Rat Anti-Mouse PD‑L2 Monoclonal Antibody (Catalog # MAB1022, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab)2 Secondary Antibody (Catalog # F0113). |
