Human TNF RI/TNFRSF1A MAb (Clone 16805) (25 UG)【科瑞奥博】

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Human TNF RI/TNFRSF1A MAb (Clone 16805) (25 UG)

货号： MAB625-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB625-SP
别名	CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
全称	Tumor Necrosis Factor Receptor I
反应种属	Human
应用	Western Blot,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human TNF RI/TNFRSF1A in ELISAs and Western blots. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) TGF-alpha, hTGF-beta 1, porcine (p) TGF-beta 1, rhTGF-beta 1, pTGF-beta 1.2, pTGF-beta 2, recombinant chicken TGF-beta 3, rhTGF-beta 3, recombinant amphibian TGF-beta 5, rhTNF-beta, or rhTNF RII is observed.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize TNF RI/TNFRSF1A-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 1-6 µg/mL in the presence of 0.3 μg/mL Recombinant Human TNF RI/TNFRSF1A, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.
来源	Monoclonal Mouse IgG₁ Clone # 16805
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7132 (Human); 21937 (Mouse); 25625 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes Sci Rep, 2016;6(0):34310. Species: Human Sample Type: CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation. Authors: Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C Mucosal Immunol, 2012;5(0):702. Species: Human Sample Type: Whole Cells Application: Neut Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK J. Immunol. Methods, 2012;378(1):72-80. Species: Human Sample Type: Serum Application: ELISA Development IRF5 is required for late-phase TNF secretion by human dendritic cells. Authors: Krausgruber T, Saliba D, Ryzhakov G Blood, 2010;115(22):4421-30. Species: Human Sample Type: Whole Cells Application: Neut Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ Transplantation, 2009;87(12):1814-20. Species: Human Sample Type: Urine Application: Antibody Array Development Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1. Authors: Lee SY, Cherla RP, Tesh VL Infect. Immun., 2007;75(3):1291-302. Species: Human Sample Type: Whole Cells Application: Functional Assay Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production. Authors: Emsley HC, Smith CJ, Gavin CM BMC Neurol, 2007;7(0):5. Species: Human Sample Type: Plasma Application: ELISA Development LIGHT (TNFSF14), a novel mediator of bone resorption, is elevated in rheumatoid arthritis. Authors: Edwards JR, Sun SG, Locklin R, Shipman CM, Adamopoulos IE, Athanasou NA, Sabokbar A Arthritis Rheum., 2006;54(5):1451-62. Species: Human Sample Type: Whole Cells Application: Neut trans fatty acids and systemic inflammation in heart failure. Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC Am. J. Clin. Nutr., 2004;80(6):1521-5. Species: Human Sample Type: Plasma Application: ELISA Development Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. Authors: Copeland S, Siddiqui J, Remick D J. Immunol. Methods, 2004;284(1):99-106. Species: Human Sample Type: Cell Culture Supernates Application: Array Development Absence of caspase 8 and high expression of PED protect primitive neural cells from cell death. Authors: Ricci-Vitiani L, Pedini F, Mollinari C, Condorelli G, Bonci D, Bez A, Colombo A, Parati E, Peschle C, De Maria R J. Exp. Med., 2004;200(10):1257-66. Species: Human Sample Type: Whole Cells Application: Flow Release of full-length 55-kDa TNF receptor 1 in exosome-like vesicles: a mechanism for generation of soluble cytokine receptors. Authors: Hawari FI, Rouhani FN, Cui X, Yu ZX, Buckley C, Kaler M, Levine SJ Proc. Natl. Acad. Sci. U.S.A., 2004;101(5):1297-302. Species: Human Sample Type: Cell Lysates Application: Flow Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1). Authors: Chopin V, Slomianny C, Hondermarck H, Le Bourhis X Exp. Cell Res., 2004;298(2):560-73. Species: Human Sample Type: Cell Lysates Application: WB Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression. Authors: Gee K, Lim W, Ma W, Nandan D, Diaz-Mitoma F, Kozlowski M, Kumar A J. Immunol., 2002;169(10):5660-72. Species: Human Sample Type: Whole Cells Application: Neut A pilot study of pentoxifylline for the treatment of primary sclerosing cholangitis. Authors: Bharucha AE, Jorgensen R, Lichtman SN, LaRusso NF, Lindor KD Am. J. Gastroenterol., 2000;95(9):2338-42. Species: Human Sample Type: Serum Application: ELISA Development Innate immunity associated with inflammatory responses and cytokine production against common dietary proteins in patients with autism spectrum disorder. Authors: Jyonouchi H, Sun S, Itokazu N Neuropsychobiology, 0;46(2):76-84. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human TNF RI/TNFRSF1A Extracellular domain
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adipocytokines Cell Structure and Signaling Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines in Lipid/Cholesterol Metabolism Diabetes Diabetic Peripheral Neuropathy EMT Induction Endothelial Cell Markers Human CD Marker Products ICC / IHC Images: TNF RI/TNFRSF1A Neuroinflammation Obesity Regulation of Apoptosis by TNF Superfamily Members T Cell Cytokine Signaling TNF Superfamily Receptors

参考图片

TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody.

Recombinant Human TNF RI/TNFRSF1A (Catalog # 636‑R1) inhibits Recombinant Human TNF‑ alpha (Catalog # 210‑TA) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TNF‑ alpha (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (0.3 μg/mL) is neutralized (green line) by increasing concentrations of Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB625). The ND50 is typically 1-6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).