Human TACI/TNFRSF13B MAb (Clone 165604) (25 UG)【科瑞奥博】

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Human TACI/TNFRSF13B MAb (Clone 165604) (25 UG)

货号： MAB1741-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1741-SP
简介	Clone 165604 was used by HLDA to establish CD designation
别名	CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B
全称	Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B
反应种属	Human
应用	Flow Cytometry,Blockade of Receptor-ligand Interaction,CyTOF-ready
目标/特异性	Detects human TACI/TNFRSF13B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human BCMA or recombinant mouse TACI is observed.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.05-0.2 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) to immobilized Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
来源	Monoclonal Mouse IgG₁ Clone # 165604
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	23495 (Human); 57916 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Macrophages express membrane bound form of APRIL that can generate immunomodulatory signals. Authors: Lee SM, Jeon ST, Suk K, Lee WH Immunology, 2010;131(3):350-6. Species: Human Sample Type: Whole Cells Application: Flow The transmembrane activator TACI triggers immunoglobulin class switching by activating B cells through the adaptor MyD88. Authors: He B, Santamaria R, Xu W, Cols M, Chen K, Puga I, Shan M, Xiong H, Bussel JB, Chiu A, Puel A, Reichenbach J, Marodi L, Doffinger R, Vasconcelos J, Issekutz A, Krause J, Davies G, Li X, Grimbacher B, Plebani A, Meffre E, Picard C, Cunningham-Rundles C, Casanova JL, Cerutti A Nat. Immunol., 2010;11(9):836-45. Species: Human Sample Type: Whole Cells Application: Flow Neutralizing B-cell activating factor antibody improves survival and inhibits osteoclastogenesis in a severe combined immunodeficient human multiple myeloma model. Authors: Neri P, Kumar S, Fulciniti MT, Vallet S, Chhetri S, Mukherjee S, Tai Y, Chauhan D, Tassone P, Venuta S, Munshi NC, Hideshima T, Anderson KC, Raje N Clin. Cancer Res., 2007;13(19):5903-9. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human TACI/TNFRSF13B Ser2-Thr166 Accession # O14836
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Xia, X.-Z.et al. (2000) J. Exp. Med. 192:137. von Bulow, G.U.et al. (1997) Science, 278:138. Gross, J.A.et al. (2000) Nature 404:995. Marsters, S.A.et al. (2000) Curr. Biol. 10:785. Yan, M.et al. (2000) Nature Immunol. 1:37. Wu, Y.et al. (2000) J. Biol. Chem. 275:35478.

参考图片

Detection of TACI/TNFRSF13B in Raji Human Cell Line by Flow Cytometry. Raji human Burkitts lymphoma cell line was stained with Mouse Anti-Human TACI/TNFRSF13B Monoclonal Antibody (Catalog # MAB1741, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by PerCP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0114).