| 货号 | MAB3542-SP |
| 别名 | BMP-7; bone morphogenetic protein 7; Eptotermin alfa; OP-1; OP-1OP1; Osteogenic protein 1 | 全称 | Bone Morphogenetic Protein 7 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | Detects human BMP-7 in ELISAs. In ELISAs, this antibody shows approximately 25-50% cross-reactivity with recombinant human (rh) BMP‑6 and no cross-reactivity with rhBMP-2, -3, -4, -5, or -8. |
| 使用方法 | Immunohistochemistry: 8-25 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize BMP‑7-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Erlacher, L. et al. (1998) J. Bone Miner. Res. 13:383. The Neutralization Dose (ND50) is typically 4-12 µg/mL in the presence of 1 µg/mL Recombinant Human BMP‑7 and 50 µg/mL L-ascorbic acid. |
| 来源 | Monoclonal Mouse IgG1 Clone # 164313 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 655 (Human); 12162 (Mouse); 85272 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Bone morphogenetic protein-6 promotes osteoblastic prostate cancer bone metastases through a dual mechanism. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human BMP‑7 Arg292-His431 Accession # P18075 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | BMP-7, or osteogenic protein 1 (OP-1), is one of at least 15structurally and functionally related BMPs, which are members of thetransforming growth factor beta (TGF-beta ) superfamily. BMPs regulatecartilage and bone formation, embryogenesis and morphogenesis ofvarious tissues and organs, and growth, differentiation, chemotaxis andapoptosis of various cell types. Biologically active BMP-7 is adisulfide-linked homodimer. Cellular responses to BMP-7 have been shownto be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Alkaline Phosphatase Production Induced by BMP‑7 and Neutralization by Human BMP‑7 Antibody. Recombinant Human BMP‑7 (Catalog # 354-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542). The ND50 is typically 4-12 µg/mL in the presence of L-ascorbic acid (50 µg/mL). | |
BMP‑7 in Human Prostate Cancer Tissue. BMP‑7 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
BMP‑7 in Human Prostate. BMP‑7 was detected in immersion fixed paraffin-embedded sections of human prostate using Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
