| 货号 | MAB1117-SP |
| 别名 | C7; chemokine (C-X-C motif) ligand 10; CRG-2; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10 |
| 反应种属 | Cotton Rat |
| 应用 | Western Blot,Neutralization |
| 目标/特异性 | Detects cotton rat CXCL10/IP‑10/CRG‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CXCL1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, or 16, recombinant mouse CXCL1, 2, 4, 6, 7, 9, 10, 11, 12, 13, 15 or 16, recombinant rat CXCL1, 2, 3, 5 or recombinant porcine CXCL8 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR3. The Neutralization Dose (ND50) is typically 20-40 µg/mL in the presence of 10 µg/mL Recombinant Cotton Rat CXCL10/IP‑10/CRG‑2. |
| 来源 | Monoclonal Mouse IgG1 Clone # 163426 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3627 (Human); 15945 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant cotton rat CXCL10/IP‑10/CRG‑2 Ile22-Val94 Accession # AAL16935 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Cotton Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | IP-10, also named mob-1 in rat and crg-2 in mouse, is a member of the non-ELR-motif containing CXC chemokine subfamily and has been designated CXCL10. It is induced in various cell types (including keratinocytes, endothelial cells, fibroblasts, smooth muscle cells, monocytes/macrophages, astrocytes, neutrophils, hepatocytes, T cells, epithelial cells and mesothelial cells) in response to stimulation by type I and/or type II interferon as well as by LPS. IP-10 has been shown to chemoattract activated CD4+ and CD8+ T cells, activated NK cells and smooth muscle cells. It also induces the adhesion of activated T cells to VCAM and ICAM. In addition, IP-10 has been reported to antagonize the angiogenic activity of FGF. IP-10 binds to and activates CXCR3, the chemokine receptor that is shared by MIG/CXCL9 and I-TAC/CXCL11, two additional non-ELR CXC chemokines that are induced by interferon. Cotton rat IP-10 cDNA encodes a 94 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 73 aa mature protein with one potential N-linked glycosylation site. Mature cotton rat IP-10 shares 66%, 64%, and 66% aa sequence identity with rat, mouse and human IP10, respectively (1‑7). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CXCL10/IP‑10/CRG‑2 and Neutralization by Cotton Rat CXCL10/IP‑10/CRG‑2 Antibody. Recombinant Cotton Rat CXCL10/IP‑10/CRG‑2 (Catalog # 1117-IP) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CXCL10/IP‑10/CRG‑2 (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti‑Cotton Rat CXCL10/ IP‑10/CRG‑2 Monoclonal Antibody (Catalog # MAB1117). The ND50 is typically 20‑40 µg/mL. |
