| 货号 | MAB1078-SP |
| 别名 | FIL1 epsilon; FIL1; FIL1(EPSILON); FIL1E; IL-1 epsilon; IL1(EPSILON); IL1E; IL-1E; IL-1F6 (FIL-1-epsilon); IL1F6; IL-1F6; IL36A; interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; Interleukin 36, Alpha; Interleukin-1 epsilon; interleukin-1 family member 6; Interleukin-36 Alpha | 全称 | Interleukin 36 alpha/Interleukin 1 Family 6 |
| 反应种属 | Human |
| 应用 | Western Blot |
| 目标/特异性 | Detects human IL‑36 alpha /IL‑1F6 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-1 receptor antagonist, rhIL-18, rhIL-36Ra, rhIL-36 beta or recombinant mouse IL-36 alpha is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 27179 (Human); 54448 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human IL‑36 alpha /IL‑1F6 Met1-Phe158 Accession # Q9UHA7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Human interleukin 1 family member #6 [IL-1F6; also IL-36 alpha and FIL-1 epsilon (epsilon)] is a member of the IL-1 family of proteins (1‑3, 6). IL-1 family members include IL-1 beta, IL-1 alpha, IL‑1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-36 alpha is synthesized as a 158 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (1‑3). It appears to be actively secreted (1). When found in cell lysate, it presents as an 18 kDa monomer (2). Human to mouse, full length IL-36 alpha has 54% aa identity. Within the family, IL-36 alpha is 30% aa identical to IL-1ra, and 27%, 31%, 36%, 46%, 57% and 28% aa identical to IL-1 beta, IL-36Ra, IL-1F7, IL-36 beta, IL-36 gamma, and IL-1F10, respectively. Cells reported to express IL-36 alpha include monocytes, B cells and T cells (1, 4). Notably, IL-36 alpha is the only novel IL-1 family member found to be expressed on T-cells. The receptor for IL-36 alpha is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL-36 alpha, along with IL-36 beta and IL-36 gamma, has been shown to act as an agonist by activating the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner. This suggests that IL-36 alpha may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|