| 货号 | MAB10522-SP |
| 别名 | CAAF1; CAAF1Neutrophil S100 protein; CAAFI; CAGC; CAGCS100 calcium binding protein A12 (calgranulin C); CAGCS100; Calcium-binding protein in amniotic fluid 1; Calgranulin C; calgranulin-C; CGRPEN-RAGE; ENRAGE; Extracellular newly identified RAGE-binding protein; MRP6; p6calgranulin C; protein S100-A12; S100 calcium binding protein A12; S100 calcium-binding protein A12 (calgranulin C); S100 calcium-binding protein A12; S100A12 | 全称 | Extracellular Newly Identified RAGE-binding Protein |
| 反应种属 | Human |
| 应用 | Western Blot |
| 目标/特异性 | Detects human EN-RAGE/S100A12 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6283 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E.coli-derived recombinant human EN-RAGE/S100A12 Met1-Glu92 Accession # NP_005612 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | EN-RAGE, also known as S100A12 and Calgranulin C, is a 10 kDa secreted molecule that belongs to the S100 family of calcium-binding proteins. EN-RAGE contains two EF-hand domains and forms dimers and hexamers in solution. EN-RAGE is overexpressed at sites of inflammation and interacts with RAGE on endothelial cells, lymphocytes, and monocytes to promote inflammatory responses. Human EN-RAGE shares 66% and 70% amino acid sequence identity with bovine and porcine EN-RAGE, respectively; mouse and rat orthologs have not been identified. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human EN‑RAGE/S100A12 by Western Blot. Western blot shows lysates of human granulocytes. PVDF Membrane was probed with 1 µg/mL of Human EN‑RAGE/S100A12 Monoclonal Antibody (Catalog # MAB10522) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EN‑RAGE/S100A12 at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
