Human EN-RAGE MAb (Clone 161205) (25 UG)【科瑞奥博】

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Human EN-RAGE MAb (Clone 161205) (25 UG)

货号： MAB10521-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB10521-SP
别名	CAAF1; CAAF1Neutrophil S100 protein; CAAFI; CAGC; CAGCS100 calcium binding protein A12 (calgranulin C); CAGCS100; Calcium-binding protein in amniotic fluid 1; Calgranulin C; calgranulin-C; CGRPEN-RAGE; ENRAGE; Extracellular newly identified RAGE-binding protein; MRP6; p6calgranulin C; protein S100-A12; S100 calcium binding protein A12; S100 calcium-binding protein A12 (calgranulin C); S100 calcium-binding protein A12; S100A12
全称	Extracellular Newly Identified RAGE-binding Protein
反应种属	Human
应用	Western Blot,Flow Cytometry,CyTOF-ready
目标/特异性	Detects recombinant human EN-RAGE in direct ELISAs.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6283 (Human)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human EN-RAGE Met1-Glu92 Accession # P80511
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	EN-RAGE, also known as S100A12 and Calgranulin C, is a 10 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of EF-hand calcium-binding proteins. Like other S100 proteins, S100A12 is small and generally acidic (1‑3). EN-RAGE forms noncovalent homodimers in the absence of divalent cations (4‑6); calcium and zinc promote the formation of higher order assemblies including tetramers and hexamers (4, 7, 8). The ability of S100A12 to chelate zinc enables it to inhibit the zinc-dependent metalloproteases MMP-2, -3, and -9 (8). S100A12 also forms heterodimers with S100A9 and binds to RAGE (Receptor for Advanced Glycation End-products), Annexin V, and several cytosolic enzymes involved in energy metabolism (9, 10). The hexameric form of EN-RAGE in particular binds RAGE with high affinity (7). EN-RAGE induces a variety of inflammatory responses including the in vivo recruitment of neutrophils, monocytes, and mast cells and the activation of mast cells and vascular endothelial cells (9, 11‑14). EN-RAGE is found at elevated levels under inflammatory conditions such as asthma, gout, rheumatoid arthritis synovial fluid, and atherosclerosis (8, 12, 14). S100A12 also promotes neurite outgrowth in isolated hippocampal neurons (15). An ortholog of S100A12 has not been identified in rodents, but the human protein is functional in mice and rats (11‑16).
运输条件	Blue Ice
存放说明	4℃
参考文献	Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201. Leclerc, E. et al. (2009) Biochim. Biophys. Acta 1793:993. Wicki, R. et al. (1996) Cell Calcium 20:459. Moroz, O.V. et al. (2009) BMC Biochem. 10:11. Miranda, L.P. et al. (2001) FEBS Lett. 488:85. Vogl, T. et al. (1999) J. Biol. Chem. 274:25291. Xie, J. et al. (2007) J. Biol. Chem. 282:4218. Goyette, J. et al. (2009) J. Immunol. 183:593. Hatakeyama, T. et al. (2004) Eur. J. Biochem. 271:3765. Hofmann, M.A. et al. (1999) Cell 97:889. Yang, Z. et al. (2001) J. Leukoc. Biol. 69:986. Rouleau, P. et al. (2003) Clin. Immunol. 107:46. Yan, W.X. et al. (2008) J. Biol. Chem. 283:13035. Yang, Z. et al. (2007) J. Allergy Clin. Immunol. 119:106. Mikkelsen, S.E. et al. (2001) J. Neurochem. 79:767. Fuellen, G. et al. (2004) OMICS 8:334.

参考图片

Detection of Human EN‑RAGE/S100A12 by Western Blot. Western blot shows lysate of human granulocytes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human EN‑RAGE/S100A12 Monoclonal Antibody (Catalog # MAB10521) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EN‑RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.