| 货号 | MAB1077-SP |
| 别名 | CD321 antigen; CD321; F11 receptor; F11R; JAM-1; JAM1CD321; JAMA; JAM-A; JCAMJAM; Junctional adhesion molecule 1Platelet F11 receptor; junctional adhesion molecule A; PAM-1; PAM-1KAT; Platelet adhesion molecule 1 | 全称 | Junctional Adhesion Molecule A |
| 反应种属 | Mouse |
| 应用 | Western Blot |
| 目标/特异性 | Detects mouse JAM-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human JAM‑A. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 50848 (Human); 16456 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse JAM-A Lys27-Ala242 Accession # O88792 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | The family of junctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-A is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (3). It is also expressed on circulating megakaryocytes. Mouse JAM-A cDNA predicts a 300 amino acid (aa) residue precursor protein with a putative 23 aa signal peptide, a 215 aa extracellular region containing two Ig-like V-subset domains, a 17 aa transmembrane domain and a 45 aa cytoplasmic domain. The human and mouse protein share approximately 67% aa sequence homology. Mouse JAM-A also shares approximately 35% aa sequence homology with mouse JAM-B or JAM-C. JAM-A exhibits homotypic interactions to regulate tight junction assembly and modulate paracellular permeability (1 - 3). The human JAM-A homotypic interation also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (4). JAM-A is involved in leukocyte adhesion and transmigration through the endothelium (3, 5). JAM-A has also been shown to bind reovirus attachment protein sigma-1 to permit reovirus infection and signal virus-induced apoptosis (6). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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