Human MICA MAb (Clone 159227) (25 UG)【科瑞奥博】

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Human MICA MAb (Clone 159227) (25 UG)

货号： MAB1300-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1300-SP
别名	FLJ60820; MGC111087; PERB11.1
全称	MHC Class I-related Protein A
反应种属	Human
应用	Western Blot,Flow Cytometry,Blockade of Receptor-ligand Interaction,CyTOF-ready
目标/特异性	Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MICB is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.02-0.06 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human MICA Fc Chimera (Catalog # 1300-MA) to immobilized Recombinant Human NKG2D Fc Chimera (Catalog # 1299-NK) coated at 2 µg/mL (100 µL/well). At 0.4 μg/mL, this antibody will block >90% of the binding.
来源	Monoclonal Mouse IgG_2B Clone # 159227
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	100507436 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells Sci Rep, 2016;6(0):32470. Species: Human Sample Type: Whole Cells Application: Flow NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. Authors: Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C J Invest Dermatol, 2015;135(5):1311-9. Species: Human Sample Type: Whole Cells Application: FACS sorting Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells. Authors: Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci M, Ricciardi M, Fuerst D, Amendola M, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A J Immunol, 2015;195(2):736-48. Species: Human Sample Type: Whole Cells Application: Neut MicroRNA editing facilitates immune elimination of HCMV infected cells. Authors: Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le V, Galun E, Horejsi V, Isakov O, Shomron N, Wolf D, Hengel H, Mandelboim O PLoS Pathog, 2014;10(2):e1003963. Species: Human Sample Type: Whole Cells Application: Flow Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1. Authors: Soriani A, Iannitto M, Ricci B, Fionda C, Malgarini G, Morrone S, Peruzzi G, Ricciardi M, Petrucci M, Cippitelli M, Santoni A J Immunol, 2014;193(2):950-60. Species: Human Sample Type: Cell Lysates Application: Flow Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation. Authors: Fielding C, Aicheler R, Stanton R, Wang E, Han S, Seirafian S, Davies J, McSharry B, Weekes M, Antrobus P, Prodhomme V, Blanchet F, Sugrue D, Cuff S, Roberts D, Davison A, Lehner P, Wilkinson G, Tomasec P PLoS Pathog, 2014;10(5):e1004058. Species: Human Sample Type: Whole Cells Application: Neut Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A. Authors: Nielsen N, Odum N, Urso B, Lanier LL, Spee P PLoS ONE, 2012;7(2):e31959. Species: Human Sample Type: Whole Cells Application: Flow NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner. Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J Lab. Invest., 2012;92(7):967-77. Species: Human Sample Type: Whole Cells Application: Flow MiR-10b Downregulates the Stress-Induced Cell Surface Molecule MICB, a Critical Ligand for Cancer Cell Recognition by Natural Killer Cells. Authors: Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O Cancer Res, 2012;72(21):5463-72. Species: Human Sample Type: Whole Cells Application: Flow Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma. Authors: Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A Oncogene, 2011;30(23):2622-32. Species: Human Sample Type: Whole Cells Application: Flow Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus. Authors: Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG J. Exp. Med., 2011;208(1):13-21. Species: Human Sample Type: Whole Cells Application: Flow Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells. Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L PLoS ONE, 2011;6(2):e16899. Species: Human Sample Type: Cell Lysates Application: WB Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A Blood, 2009;113(13):2955-64. Species: Human Sample Type: Whole Cells Application: Neut NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways. Authors: Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC Mol. Immunol., 2008;45(15):3917-25. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	BaF3 mouse pro-B cell line transfected with human MICA
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.
运输条件	Blue Ice
存放说明	4℃
参考文献	Groh, V. et al. (2001) Nature Immunol. 2:255. Stephens, H. (2001) Trends Immunol. 22:378. Bauer, S. et al. (1999) Science 285:727. Groh, V. et al. (2002) Nature 419:734. Steinle, A. et al. (2001) Immunogenetics 53:279. Pende, D. et al. (2002) Cancer Res. 62:6178. NKG2D and its Ligands (2002) http://www.RnDSystems.com

参考图片

Detection of MICA in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA Monoclonal Antibody (Catalog # MAB1300, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).