Human MICA/B MAb (Clone 159207) (25 UG)【科瑞奥博】

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Human MICA/B MAb (Clone 159207) (25 UG)

货号： MAB13001-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB13001-SP
别名	MHC class I chain-related protein A; MHC class I chain-related protein B; MIC; MICA; MICB; PERB11; PERB11.1; PERB11.2
全称	MHC Class I-related Protein A and B
反应种属	Human
应用	Flow Cytometry,Immunohistochemistry,Blockade of Receptor-ligand Interaction,CyTOF-ready
目标/特异性	Detects human MICA/B in direct ELISAs.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 8-25 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.03-0.1 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Biotinylated Human MICA Fc Chimera to immobilized Recombinant Human NKG2D Fc Chimera (Catalog # 1299‑NK) coated at 2 µg/mL (100 µL/well). At 0.4 μg/mL, this antibody will block >90% of the binding.
来源	Monoclonal Mouse IgG_2A Clone # 159207
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4276 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity. Authors: Cox S, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal J, Saudemont A Eur J Immunol, 2015;45(8):2324-34. Species: Human Sample Type: Plasma Application: IP Hepatitis C virus impairs natural killer cell-mediated augmentation of complement synthesis. Authors: Kim H, Bose S, Meyer K, Ray R J Virol, 2014;88(5):2564-71. Species: Human Sample Type: Cell Lysates Application: WB Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma. Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S PLoS Pathog., 2012;8(1):e1002486. Species: Human Sample Type: Whole Cells Application: Flow Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma. Authors: Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A Oncogene, 2011;30(23):2622-32. Species: Human Sample Type: Whole Cells Application: Flow Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells. Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L PLoS ONE, 2011;6(2):e16899. Species: Human Sample Type: Cell Lysates Application: WB Transfer of NKG2D and MICB at the cytotoxic NK cell immune synapse correlates with a reduction in NK cell cytotoxic function. Authors: Roda-Navarro P, Vales-Gomez M, Chisholm SE, Reyburn HT Proc. Natl. Acad. Sci. U.S.A., 2006;103(30):11258-63. Species: Human Sample Type: Whole Cells Application: ICC Cancer cells become susceptible to natural killer cell killing after exposure to histone deacetylase inhibitors due to glycogen synthase kinase-3-dependent expression of MHC class I-related chain A and B. Authors: Skov S, Pedersen MT, Andresen L, Straten PT, Woetmann A, Odum N Cancer Res., 2005;65(23):11136-45. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	BaF3 mouse pro-B cell line transfected with human MICA Accession # Q29983
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	MICA and MICB are stress-induced type I transmembrane proteins thatfunction as ligands for NKG2D. They are distantly related to MHC classI proteins and are expressed on epithelial tumors and in response toinfections.
运输条件	Blue Ice
存放说明	4℃
参考文献	ICC / IHC Images: MICA/B Mini-review: NKG2D and its Ligands Natural Killer T (NKT) Cells

参考图片

Detection of MICA/B in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA/B Monoclonal Antibody (Catalog # MAB13001, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

MICA/B in Human Pancreas. MICA/B was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human MICA/B Monoclonal Antibody (Catalog # MAB13001) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.