| 货号 | MAB9372-SP |
| 别名 | 4-1BB ligand receptor; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9 |
| 反应种属 | Mouse |
| 应用 | Western Blot,Blockade of Receptor-ligand Interaction |
| 目标/特异性 | Detects mouse 4-1BB in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) BAFF R, rmCD27, rmCD30, rmCD40, rmDR3, rmEDAR, rmFas, rmGITR, rmLymphotoxin beta R, rmNGF R, rmOPG, rmOX40, rmRANK, rmTAJ, rmTNF RI, rmTNF RII, rh4-1BB, rhDR6, or rhHVEM. |
| 使用方法 | Western Blot: 1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.3 to 1.5 µg/mL of this antibody will block 50% of the binding of 2 ng/mL of biotinylated Recombinant Mouse 4-1BB Ligand to immobilized Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera (Catalog # 937-4B) coated at 1 µg/mL (100 μL/well). At 20 µg/mL, this antibody will block >90% of the binding. |
| 来源 | Monoclonal Rat IgG2B Clone # 158306 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse 4-1BB Val24-Leu187 Accession # P20334 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | 4-1BB, also known as CD137 and ILA (induced by lymphocyte activation), is a TNF receptor superfamily member and has been designated TNFRSF9. Mouse 4-1BB cDNA encodes a 256 amino acid (aa) residues type I transmembrane protein with a putative 23 aa signal peptide, a 164 aa extracellular domain, a 21 aa transmembrane domain and a 48 aa cytoplasmic region (1 - 3). A soluble 4‑1BB is released from surfaces of cells expressing the transmembrane protein (4). Mouse 4‑1BB shares approximately 60% aa sequence identity with its human counterpart. 4‑1BB is expressed on activated CD4+ and CD8+ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils (5). The ligand for 4-1BB (4-1BBL), also named TNFSF9, belongs to the TNF ligand superfamily. 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines. In response to 4-1BBL binding, 4-1BB transduce a T cell costimulatory signal in both CD4+ and CD8+ T cells to promote survival and enhance proliferation, cytokine production and effector function. In vivo, the costimulatory activity of 4-1BB has been shown to be important in graft-vs-host disease and antiviral CTL responses. On dendritic cells, 4-1BB is a DC-activating molecules that enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules, resulting in an improved ability to stimulate T cell responses (1 - 5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
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