| 货号 | MAB5041-SP |
| 别名 | B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra | 全称 | Interleukin 4 |
| 反应种属 | Rat |
| 应用 | Western Blot,Neutralization |
| 目标/特异性 | Detects recombinant rat IL-4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human IL-4, recombinant mouse IL-4, recombinant feline IL-4, recombinant canine IL-4, recombinant porcine IL-4, recombinant cotton rat IL-4, or recombinant mouse IL-13 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize IL‑4-induced proliferation in the rat splenocytes. The Neutralization Dose (ND50) is typically 0.8-4.0 µg/mL in the presence of 4 ng/mL Recombinant Rat IL‑4 and 10 µg/mL PHA. |
| 来源 | Monoclonal Mouse IgG1 Clone # 154101 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Development of inflammatory bowel disease in Long-Evans Cinnamon rats based on CD4+CD25+Foxp3+ regulatory T cell dysfunction. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant rat IL‑4 Cys25-Ser147 Accession # P20096.2 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Rat IL-4 is synthesized with a 24 aa signal sequence. Mature rat IL-4 shares 41%, 43%, and 59% aa sequence identity with bovine, human, and mouse IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5‑7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in rodent B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑4 and Neutralization by Rat IL‑4 Antibody. Recombinant Rat IL‑4 (Catalog # 504-RL) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑4 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL‑4 Monoclonal Antibody (Catalog # MAB5041). The ND50 is typically 0.8‑4.0 µg/mL in the presence of PHA (10 µg/mL). |
