Porcine IFN-gamma MAb (Clone 154002) (25 UG)【科瑞奥博】

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Porcine IFN-gamma MAb (Clone 154002) (25 UG)

货号： MAB9851-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB9851-SP
别名	IFG; IFI; IFNG; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
全称	Interferon gamma
反应种属	Porcine
应用	Neutralization
目标/特异性	Detects porcine IFN-gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant IFN-gamma from human, mouse, rat, dog, cat, or cotton rat is observed.
使用方法	Neutralization: Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the PK‑15 porcine kidney epithelial cell line. The Neutralization Dose (ND₅₀) is typically 15-60 ng/mL in the presence of 0.2 ng/mL Recombinant Porcine IFN‑ gamma.
来源	Monoclonal Mouse IgG_2B Clone # 154002
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant porcine IFN-gamma Ser21-Lys166 Accession # P17803
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Porcine
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature porcine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 72%‑79% amino acid sequence identity with bovine, canine, equine, and feline IFN-gamma and 41%‑57% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, anti-proliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
运输条件	Blue Ice
存放说明	4℃
参考文献	Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. Pestka, S. et al. (2004) Immunol. Rev. 202:8. Dijkmans, R. et al. (1990) Nucl. Acids Res. 18:4259. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

参考图片

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Porcine IFN‑ gamma Antibody.
Recombinant Porcine IFN‑ gamma (Catalog # 985‑PI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the PK‑15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Porcine IFN‑ gamma (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IFN‑ gamma Monoclonal Antibody (Catalog # MAB9851). The ND₅₀ is typically 15‑60 ng/mL.