| 货号 | MAB1035-SP |
| 别名 | IL-10C; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19 | 全称 | Interleukin 19 |
| 反应种属 | Human |
| 应用 | CyTOF-ready,Intracellular Staining by Flow Cytometry,Neutralization |
| 目标/特异性 | Detects human IL-19 in direct ELISAs. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 2.5 µg/106cells Neutralization: Measured by its ability to neutralize IL‑19-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND50) is typically 0.2-0.5 µg/mL in the presence of 3 ng/mL Recombinant Human IL‑19. |
| 来源 | Monoclonal Mouse IgG2B Clone # 152112 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 29949 (Human); 329244 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human IL-19 Leu25-Ala177 Accession # AAN40906 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. Its gene contains two alternate translation initiation sites, generating precursors of 215 amino acids (aa) and 177 aa, respectively. Both isoforms are processed to 17 kDa, 153 aa mature molecules. IL-19 contains seven helices and is secreted as a 35 kDa monomer. There are two potential N-linked glycosylation sites, and it is likely that the molecule is glycosylated. Mature human IL-19 shares 69% aa sequence identity with the mature mouse homologue. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is limited to activated keratinocytes and monocytes. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R alpha, also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R beta or IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Functionally, IL-19 induces IL-6 and TNF-alpha production by monocytes, and drives T-helper cell differentiation towards a Th2 response (1‑5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑19 and Neutralization by Human IL‑19 Antibody. Recombinant Human IL‑19 (Catalog # 1035-IL) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta in a dose‑dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑19 (3 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑19 Monoclonal Antibody (Catalog # MAB1035). The ND50 is typically 0.2‑0.5 µg/mL. |
