Human CCR7 MAb (Clone 150503) (25 UG)【科瑞奥博】

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Human CCR7 MAb (Clone 150503) (25 UG)

货号： MAB197-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB197-SP
简介	Clone 150503 was used by HLDA to establish CD designation
别名	BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7chemokine (C-C) receptor 7; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor
反应种属	Human
应用	Flow Cytometry,CyTOF-reported,Immunocytochemistry,Neutralization
目标/特异性	Detects human CCR7.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR7. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 50 ng/mL Recombinant Human CCL19/MIP‑3 beta.
来源	Monoclonal Mouse IgG_2A Clone # 150503
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1236 (Human); 12775 (Mouse); 287673 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Dengue virus infection induces interferon-lambda1 to facilitate cell migration Sci Rep, 2016;6(0):24530. Species: Human Sample Type: Whole Cells Application: Flow Heightened Immune Activation in Fetuses with Gastroschisis May Be Blocked by Targeting IL-5 J Immunol, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Flow Compartmentalization of Total and Virus-Specific Tissue-Resident Memory CD8+ T Cells in Human Lymphoid Organs PLoS Pathog, 2016;12(8):e1005799. Species: Human Sample Type: Whole Cells Application: Flow Enhanced Chemokine Receptor Recycling and Impaired S1P1 Expression Promote Leukemic Cell Infiltration of Lymph Nodes in Chronic Lymphocytic Leukemia. Authors: Patrussi L, Capitani N, Martini V, Pizzi M, Trimarco V, Frezzato F, Marino F, Semenzato G, Trentin L, Baldari C Cancer Res, 2015;75(19):4153-63. Species: Human Sample Type: Whole Cells Application: Flow Trypsin, Tryptase, and Thrombin Polarize Macrophages towards a Pro-Fibrotic M2a Phenotype. Authors: White M, Gomer R PLoS ONE, 2015;10(9):e0138748. Species: Human Sample Type: Whole Cells Application: IHC Not Specified Prognostic markers for patient outcome following vaccination with multiple MHC Class I/II-restricted WT1 peptide-pulsed dendritic cells plus chemotherapy for pancreatic cancer. Authors: Takakura K, Koido S, Kan S, Yoshida K, Mori M, Hirano Y, Ito Z, Kobayashi H, Takami S, Matsumoto Y, Kajihara M, Misawa T, Okamoto M, Sugiyama H, Homma S, Ohkusa T, Tajiri H Anticancer Res, 2015;35(1):555-62. Species: Human Sample Type: Whole Cells Application: Flow Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry. Authors: Mei H, Leipold M, Schulz A, Chester C, Maecker H J Immunol, 2015;194(4):2022-31. Species: Human Sample Type: Whole Cells Application: CyTof Effect of IL-7 Therapy on Naive and Memory T Cell Homeostasis in Aged Rhesus Macaques. Authors: Okoye A, Rohankhedkar M, Konfe A, Abana C, Reyes M, Clock J, Duell D, Sylwester A, Sammader P, Legasse A, Park B, Axthelm M, Nikolich-Zugich J, Picker L J Immunol, 2015;195(9):4292-305. Species: Primate - Macaca mulatta Sample Type: Whole Cells Application: Flow IL-21 promotes the expansion of CD27+ CD28+ tumor infiltrating lymphocytes with high cytotoxic potential and low collateral expansion of regulatory T cells. Authors: Santegoets S, Turksma A, Suhoski M, Stam A, Albelda S, Hooijberg E, Scheper R, van den Eertwegh A, Gerritsen W, Powell D, June C, de Gruijl T J Transl Med, 2015;11(0):37. Species: Human Sample Type: Whole Cells Application: Flow Tissue Distribution of Memory T and B Cells in Rhesus Monkeys following Influenza A Infection. Authors: Pichyangkul S, Yongvanitchit K, Limsalakpetch A, Kum-Arb U, Im-Erbsin R, Boonnak K, Thitithayanont A, Jongkaewwattana A, Wiboon-ut S, Mongkolsirichaikul D, Mahanonda R, Spring M, Chuang I, Mason C, Saunders D J Immunol, 2015;195(9):4378-86. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: Flow Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis. Authors: Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A Nat Commun, 2014;5(0):4715. Species: Human Sample Type: Whole Cells Application: Blocking Cytokine production and antigen recognition by human mucosal homing conjunctival effector memory CD8+ T cells. Authors: Williams G, Pachnio A, Long H, Rauz S, Curnow S Invest Ophthalmol Vis Sci, 2014;55(12):8523-30. Species: Human Sample Type: Whole Cells Application: Flow Oxygen regulates human cytotrophoblast migration by controlling chemokine and receptor expression. Authors: Schanz A, Red-Horse K, Hess A, Baston-Bust D, Heiss C, Krussel J Placenta, 2014;35(12):1089-94. Species: Human Sample Type: Cell Lysates Application: WB Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice. Authors: Antsiferova O, Muller A, Ramer P, Chijioke O, Chatterjee B, Raykova A, Planas R, Sospedra M, Shumilov A, Tsai M, Delecluse H, Munz C PLoS Pathog, 2014;10(8):e1004333. Species: Human Sample Type: Whole Cells Application: Flow NK cells from malignant pleural effusions are not anergic but produce cytokines and display strong antitumor activity on short-term IL-2 activation. Authors: Vacca P, Martini S, Garelli V, Passalacqua G, Moretta L, Mingari M Eur J Immunol, 2013;43(2):550-61. Species: Human Sample Type: whole cells Application: Flow Anti-CCR7 therapy exerts a potent anti-tumor activity in a xenograft model of human mantle cell lymphoma. Authors: Somovilla-Crespo B, Alfonso-Perez M, Cuesta-Mateos C, Carballo-de Dios C, Beltran A, Terron F, Perez-Villar J, Gamallo-Amat C, Perez-Chacon G, Fernandez-Ruiz E, Zapata J, Munoz-Calleja C J Hematol Oncol, 2013;6(0):89. Species: Human Sample Type: Whole Cells Application: Neut Accelerated heterologous adenovirus prime-boost SIV vaccine in neonatal rhesus monkeys. Oncogene, 2012;86(15):7829-35. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: Flow Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Nature, 2012;484(7395):514-8. Species: Human Sample Type: Whole Cells Application: Flow Identification of human NK17/NK1 cells. Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA PLoS ONE, 2011;6(10):e26780. Species: Human Sample Type: Whole Cells Application: Flow Blocking of alpha4beta7 gut-homing integrin during acute infection leads to decreased plasma and gastrointestinal tissue viral loads in simian immunodeficiency virus-infected rhesus macaques. Authors: Ansari AA, Reimann KA, Mayne AE, Takahashi Y, Stephenson ST, Wang R, Wang X, Li J, Price AA, Little DM, Zaidi M, Lyles R, Villinger F J. Immunol., 2011;186(2):1044-59. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: Flow Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Authors: Islam SA, Chang DS, Colvin RA Nat. Immunol., 2011;12(2):167-77. Species: Human Sample Type: Whole Cells Application: Flow Early Induction of Polyfunctional Simian Immunodeficiency Virus (SIV)-Specific T Lymphocytes and Rapid Disappearance of SIV from Lymph Nodes of Sooty Mangabeys during Primary Infection. Authors: Meythaler M, Wang Z, Martinot A, Pryputniewicz S, Kasheta M, McClure HM, ONeil SP, Kaur A J. Immunol., 2011;186(9):5151-61. Species: Primate â€“ Cercocebus torquatus (Sooty Mangabey) Sample Type: Whole Cells Application: Flow Elevated frequencies of highly activated CD4+ T cells in HIV+ patients developing immune reconstitution inflammatory syndrome. Authors: Antonelli LR, Mahnke Y, Hodge JN, Porter BO, Barber DL, DerSimonian R, Greenwald JH, Roby G, Mican J, Sher A, Roederer M, Sereti I Blood, 2010;116(19):3818-27. Species: Human Sample Type: Whole Cells Application: Flow CD94 surface density identifies a functional intermediary between the CD56bright and CD56dim human NK-cell subsets. Authors: Yu J, Mao HC, Wei M, Hughes T, Zhang J, Park IK, Liu S, McClory S, Marcucci G, Trotta R, Caligiuri MA Blood, 2010;115(2):274-81. Species: Human Sample Type: Whole Cells Application: Flow Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques. Authors: Bolton DL, Minang JT, Trivett MT, Song K, Tuscher JJ, Li Y, Piatak M, OConnor D, Lifson JD, Roederer M, Ohlen C J. Immunol., 2010;184(1):303-14. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: Flow Distribution, persistence, and efficacy of adoptively transferred central and effector memory-derived autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones in rhesus macaques during acute infection. Authors: Minang JT, Trivett MT, Bolton DL, Trubey CM, Estes JD, Li Y, Smedley J, Pung R, Rosati M, Jalah R, Pavlakis GN, Felber BK, Piatak M, Roederer M, Lifson JD, Ott DE, Ohlen C J. Immunol., 2010;184(1):315-26. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: Flow Expression of podoplanin and other lymphatic markers in the human anterior eye segment. Authors: Birke K, Lutjen-Drecoll E, Kerjaschki D, Birke MT Invest. Ophthalmol. Vis. Sci., 2010;51(1):344-54. Species: Human Sample Type: Whole Tissue Application: IHC CCL21 overexpressed on lymphatic vessels drives thymic hyperplasia in myasthenia. Authors: Berrih-Aknin S, Ruhlmann N, Bismuth J, Cizeron-Clairac G, Zelman E, Shachar I, Dartevelle P, de Rosbo NK, Le Panse R Ann. Neurol., 2009;66(4):521-31. Species: Human Sample Type: Whole Cells Application: Flow RNA-loaded CD40-activated B cells stimulate antigen-specific T-cell responses in dogs with spontaneous lymphoma. Authors: Mason NJ, Coughlin CM, Overley B, Cohen JN, Mitchell EL, Colligon TA, Clifford CA, Zurbriggen A, Sorenmo KU, Vonderheide RH Gene Ther., 2008;15(13):955-65. Species: Canine Sample Type: Whole Cells Application: Flow Post-apoptotic tumors are more palatable to dendritic cells and enhance their antigen cross-presentation activity. Authors: Brusa D, Garetto S, Chiorino G, Scatolini M, Migliore E, Camussi G, Matera L Vaccine, 2008;26(50):6422-32. Species: Human Sample Type: Whole Cells Application: Flow Idiopathic pulmonary fibrosis fibroblasts migrate and proliferate to CC chemokine ligand 21. Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Evanoff H, Flaherty KR, Martinez FJ, Toews GB, Hogaboam CM Eur. Respir. J., 2007;29(6):1082-93. Species: Human Sample Type: Cell Lysates Application: WB Vitamins A and D are potent inhibitors of cutaneous lymphocyte-associated antigen expression. Authors: Yamanaka K, Dimitroff CJ, Fuhlbrigge RC, Kakeda M, Kurokawa I, Mizutani H, Kupper TS J. Allergy Clin. Immunol., 2007;121(1):148-157.e3. Species: Human Sample Type: Whole Cells Application: Flow Transduction with human telomerase reverse transcriptase immortalizes a rhesus macaque CD8+ T cell clone with maintenance of surface marker phenotype and function. Authors: Andersen H, Barsov EV, Trivett MT, Trubey CM, Giavedoni LD, Lifson JD, Ott DE, Ohlen C AIDS Res. Hum. Retroviruses, 2007;23(3):456-65. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: Flow Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH J. Immunol., 2007;179(6):3724-33. Species: Human Sample Type: Whole Cells Application: Flow Autologous chemotaxis as a mechanism of tumor cell homing to lymphatics via interstitial flow and autocrine CCR7 signaling. Authors: Shields JD, Fleury ME, Yong C, Tomei AA, Randolph GJ, Swartz MA Cancer Cell, 2007;11(6):526-38. Species: Human Sample Type: Cell Lysates Application: WB Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice. Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM Am. J. Pathol., 2007;170(4):1152-64. Species: Human Sample Type: human cell In Vivo in mice Application: Neut Prolonged (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate-driven antimicrobial and cytotoxic responses of pulmonary and systemic Vgamma2Vdelta2 T cells in macaques. Authors: Ali Z, Shao L, Halliday L, Reichenberg A, Hintz M, Jomaa H, Chen ZW J. Immunol., 2007;179(12):8287-96. Species: Primate â€“ Macaca fascicularis (Cynomolgus/Crab-eating Monkey) Sample Type: Whole Cells Application: Flow Progressive CD4+ central memory T cell decline results in CD4+ effector memory insufficiency and overt disease in chronic SIV infection. Authors: Okoye A, Meier-Schellersheim M, Brenchley JM, Hagen SI, Walker JM, Rohankhedkar M, Lum R, Edgar JB, Planer SL, Legasse A, Sylwester AW, Piatak M, Lifson JD, Maino VC, Sodora DL, Douek DC, Axthelm MK, Grossman Z, Picker LJ J. Exp. Med., 2007;204(9):2171-85. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: Flow The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors. Authors: Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS J. Immunol., 2006;177(7):4488-94. Species: Human Sample Type: Whole Cells Application: Flow Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy. Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK Int. J. Cancer, 2006;118(9):2147-57. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded The monoclonal antibody CHO-131 identifies a subset of cutaneous lymphocyte-associated antigen T cells enriched in P-selectin-binding cells. Authors: Ni Z, Campbell JJ, Niehans G, Walcheck B J. Immunol., 2006;177(7):4742-8. Species: Human Sample Type: Whole Cells Application: Flow Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi. Authors: Humphreys TL, Baldridge LA, Billings SD, Campbell JJ, Spinola SM Infect. Immun., 2005;73(7):3896-902. Species: Human Sample Type: Whole Cells Application: Flow Developing neonatal rabbit appendix, a primary lymphoid organ, is seeded by immature blood-borne B cells: evidence for roles for CD62L/PNAd, CCR7/CCL21, alpha4beta1 and LFA-1. Authors: Sinha RK, Mage RG Dev. Comp. Immunol., 2004;28(7):829-41. Species: Rabbit Sample Type: Whole Cells Application: Flow Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis. Authors: Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M Int. J. Oncol., 2004;25(3):605-13. Species: Human Sample Type: Whole Cells Application: ICC Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression. Authors: Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G Eur. J. Immunol., 2004;34(12):3485-96. Species: Human Sample Type: Whole Cells Application: Flow Evasion from NK cell immunity by MHC class I chain-related molecules expressing colon adenocarcinoma. Authors: Doubrovina ES, Doubrovin MM, Vider E, Sisson RB, O&amp;apos;Reilly RJ, Dupont B, Vyas YM J. Immunol., 2003;171(12):6891-9. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Human CCR7 transfectants Met1-Pro378 Accession # AAA58615
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCR7 is a 7 transmembrane G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Biomarkers CC Chemokines and Receptors Chemokine Receptors Chemokines and Receptors in Angiogenesis Chemokines and Receptors in Tfh Cell Differentiation and Function Dendritic Cell Adhesion and Migration Human CD Marker Products ICC / IHC Images: CCR7 Regulatory T Cells (Tregs) T Cell Migration/Adhesion Technical Notes: CCR7

参考图片

CCR7 in Human PBMCs.

CCR7 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 25 µg/mL Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of CCR7 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197) or (B) Mouse IgG_2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Human CCR7 Antibody.

Recombinant Human CCL19/MIP‑3 beta (Catalog # 361‑MI) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP‑3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCR7 Monoclonal Antibody (Catalog # MAB197). The ND₅₀ is typically 1-5 µg/mL.