| 货号 | MAB9881-SP |
| 别名 | C21orf43; CD322 antigen; CD322; JAM2; JAMB; JAM-BJAM-IT/VE-JAM; junctional adhesion molecule 2JAM-2; junctional adhesion molecule B; PRO245; Vascular endothelial junction-associated molecule; VE-JAM; VE-JAMVEJAMCD322 | 全称 | Junctional Adhesion Molecule B |
| 反应种属 | Mouse |
| 应用 | Neutralization |
| 目标/特异性 | Detects mouse JAM‑B/VE-JAM in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human JAM-1, -2, or -3 is observed. |
| 使用方法 | Neutralization: Measured by its ability to neutralize JAM‑B/VE‑JAM-mediated adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line. Fong, S. et al. (2002) J. Immunol. 168:1618. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse JAM‑B/VE‑JAM Fc Chimera. |
| 来源 | Monoclonal Rat IgG2A Clone # 150005 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 58494 (Human); 67374 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Migration of encephalitogenic CD8 T cells into the central nervous system is dependent on the alpha4beta1-integrin. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse JAM‑B/VE-JAM Phe29-Asn236 (predicted) Accession # Q9JI59 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. JAM-B, alternatively named vascular endothelial JAM (VE-JAM), is expressed prominently on high endothelial venules of lymphoid organs where it is localized to the intercellular boundaries of high endothelial cells. It is also expressed on the endothelium of a variety of non-lymphoid organs, especially the heart and placenta (2, 3, 5). Mouse JAM-B/VE-JAM cDNA predicts a 298 amino acid (aa) precursor protein with a putative 28 aa signal peptide, a 209 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 38 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (2, 3). Mouse JAM-B shares approximately 79% aa sequence homology with its human homologue. It also shares approximately 35% aa sequence homology with mouse JAM-A or JAM‑C. JAM-B exhibits homotypic interactions, as well as heterotypic interactions with JAM‑C, but not JAM-A (4, 5, 7). It is also a ligand for the Integrin alpha4beta1. However, the JAM-B/alpha4beta1 interaction is facilitated only after prior adhesion of JAM-B to JAM‑C (6). Through its heterotypic interactions with JAM‑C, JAM-B is an adhesive ligand for T, NK, and dendritic cells, and may play a role in regulating leukocyte transmigration (5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Cell Adhesion Mediated by JAM‑B/VE‑JAM and Neutralization by Mouse JAM‑B/ VE‑JAM Antibody. Recombinant Mouse JAM‑B/VE‑JAM Fc Chimera (Catalog # 988-VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G‑102‑C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse JAM‑B/VE‑JAM Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse JAM-B/ VE-JAM Monoclonal Antibody (Catalog # MAB9881). The ND50 is typically 0.1-0.5 µg/mL. |
