| 货号 | MAB7151-SP |
| 别名 | BMP-5; bone morphogenetic protein 5; MGC34244 | 全称 | Bone Morphogenetic Protein 5 |
| 反应种属 | Human |
| 应用 | Neutralization |
| 目标/特异性 | Detects human BMP-5 in direct ELISAs and Western blots. In direct ELISAs, 50% cross-reactivity with recombinant human (rh) BMP-6 is observed and no cross-reactivity with rhBMP-2, -3, -4, or -7 is observed. |
| 使用方法 | Neutralization: Measured by its ability to neutralize BMP‑5-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Asahina, I. et al. (1996) Exp. Cell Res. 222:38. The Neutralization Dose (ND50) is typically 2‑10 µg/mL in the presence of 1.5 µg/mL Recombinant Human BMP‑5. |
| 来源 | Monoclonal Mouse IgG2B Clone # 149107 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 653 (Human); 12160 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human BMP‑5 Asn313-His454 Accession # P22003 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Bone Morphogenetic Protein-5 (BMP-5) is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor beta (TGF-beta ) superfamily (1). BMP-5 is synthesized as a 454 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 20 kDa C-terminal mature protein (2). Mature BMP-5 contains seven conserved cysteine residues involved in formation of the cysteine knot and the single interchain disulfide bond. Biologically active BMP-5 is a disulfide-linked homodimer of the C-terminal mature protein. Mature human BMP-5 shares 96% aa sequence identity with mouse and rat BMP-5. Cellular responses to BMP-5 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (1). BMP-5 is expressed by chondrocytes in proliferating and hypertrophic zones of bone growth plates (3). It contributes to limb development by promoting proliferation and differentiation of chondrocytes as well as apoptosis of undifferentiated mesoderm (3, 4). Genetic defects in BMP-5 which cause C-terminal truncation or loss of the proteolytic cleavage site result in multiple skeletal abnormalities, including the short ear phenotype in mice (5, 6). BMP-5 is also expressed by ovarian granulosa cells where it functions as an autocrine factor to promote GC proliferation and inhibit their production of progesterone (7). In the nervous system, BMP-5 promotes dendrite outgrowth and dopaminergic neuronal differentiation (8, 9). It is upregulated in oral squamous carcinoma cells and induces the apoptosis of some myeloma cell lines (10, 11). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Alkaline Phosphatase Production Induced by BMP‑5 and Neutralization by Human BMP‑5 Antibody. Recombinant Human BMP‑5 (Catalog # 615‑BMC) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑5 (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP‑5 Monoclonal Antibody (Catalog # MAB7151). The ND50 is typically 2-10 µg/mL. |
