| 货号 | MAB196-SP |
| 别名 | desert hedgehog (Drosophila) homolog; desert hedgehog homolog; desert hedgehog protein; desert hedgehog; DHH; HHG-3MGC35145 |
| 反应种属 | Mouse |
| 应用 | Western Blot |
| 目标/特异性 | Detects mouse Desert Hedgehog/Dhh C-Terminus in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Dhh N-terminal peptide (aa 23‑198), rmShh (aa 199‑437), rmShh (aa 25‑198), or rmIhh (aa 241‑499) is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 50846 (Human); 13363 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse Desert Hedgehog/Dhh C-Terminus Cys199-Gly396 Accession # Q61488 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are synthesized as 45 kDa precursors that are cleaved autocatalytically. The 19 kDa N-terminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Binding of this fragment to Patched receptors results in the loss of Patched repression of Smoothened signaling (1‑4). Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip) (5). Within the N-terminal peptide, mouse Dhh shares 97% and 100% amino acid (aa) sequence identity with human and rat Dhh, respectively. It shares 74% aa seqeuence identity with mouse Indian (Ihh) and Sonic hedgehog (Shh) (6, 7). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis (8, 9). It induces steroidogenic factor 1 which is instrumental in promoting Leydig cell differentiation (10, 11). It also promotes the deposition of basal lamina surrounding seminiferous tubules (8). In humans, mutations of Dhh are associated with pure gonadal dysgenesis (12). Dhh is expressed in the female by ovarian granulosa cells and the corpus luteum (13). Its up‑regulation in human ovarian cancer correlates positively with proliferative index and negatively with prognosis (14). Dhh is also expressed by Schwann cells and is up‑regulated following nerve injury (15, 16). It induces the expression of Patched and Hip in nerve fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves (15). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|