Mouse CCL1/TCA-3 MAb (Clone 148113) (25 UG)【科瑞奥博】

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Mouse CCL1/TCA-3 MAb (Clone 148113) (25 UG)

货号： MAB845-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB845-SP
别名	C-C motif chemokine 1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3
反应种属	Mouse
应用	Western Blot,Neutralization
目标/特异性	Detects mouse CCL1 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant cottonrat CCL3, 4, 5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17,18, 19, 20, 21, 23, 24, 25, 26, 27, 28, recombinant mouse CCL2, 3, 4,5,6, 7, 8,9/10/MIP-1 gamma, 11,12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or recombinant rat CCL20.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR8. The Neutralization Dose (ND₅₀) is typically 2-10 µg/mL in the presence of 0.01 µg/mL Recombinant Mouse CCL1/I‑309/TCA‑3.
来源	Monoclonal Rat IgG_2A Clone # 148113
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6346 (Human); 20290 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Authors: Islam SA, Chang DS, Colvin RA Nat. Immunol., 2011;12(2):167-77. Species: Mouse Sample Type: In Vivo Application: In vivo Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway. Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM Am. J. Respir. Crit. Care Med., 2009;179(9):772-81. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions. Authors: Yang L, Mosmann T Eur. J. Immunol., 2004;34(6):1617-26. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions. Authors: Mosmann T Eur. J. Immunol., 2004;34(6):1617-26. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse CCL1/I-309/TCA-3 Lys24-Cys92 Accession # P10146
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Mouse CCL1, also known as TCA-3, is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5 flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines. CCL1 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of CCL1 contains one potential N-linked glycosylation site. Mouse CCL1 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta and JE. CCL1 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. CCL1 has been shown to chemoattract T cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Goya, I.et al. (1998) J. Immunol. 160:1975. Burd, P.et al. (1987) J. Immunol. 139:3126.

参考图片

Chemotaxis Induced by CCL1/ITCA‑3 and Neutralization by Mouse CCL1/ITCA‑3 Antibody. Recombinant Mouse CCL1/TCA‑3 (Catalog # 845-TC) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/TCA‑3 (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL1/TCA‑3 Monoclonal Antibody (Catalog # MAB845). The ND₅₀ is typically 2-10 µg/mL.