| 货号 | MAB4281-SP |
| 别名 | C-C motif chemokine 12; chemokine (C-C motif) ligand 12; MCP-5; Scya12 |
| 反应种属 | Mouse |
| 应用 | Neutralization |
| 目标/特异性 | Detects mouse CCL12/MCP‑5 in direct ELISAs. |
| 使用方法 | Neutralization: Measured by its ability to neutralize CCL12/MCP‑5-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse CCL12/MCP‑5. |
| 来源 | Monoclonal Rat IgG2B Clone # 147801 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 20293 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse CCL12/MCP‑5 Gly23-Gly104 Accession # Q62401 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CCL12, also called MCP-5, is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. Mouse CCL12 encodes a 104 amino acid (aa) precursor protein with a 22 aa signal sequence that is cleaved to generate a 82 aa mature protein. Recombinant CCL12 is a potent chemoattractant for monocytes and lymphocytes but not neutrophils. CCL12 has been found to be a functional ligand for CCR2. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CCL12/MCP‑5 and Neutralization by Mouse CCL12/ MCP‑5 Antibody. Recombinant Mouse CCL12/MCP‑5 (Catalog # 428‑P5) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL12/MCP‑5 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL12/MCP‑5 Monoclonal Antibody (Catalog # MAB4281). The ND50 is typically 0.5-2.5 µg/mL. |
