Mouse CD3 epsilon MAb (Clone 145-2C11) (25 UG)【科瑞奥博】

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Mouse CD3 epsilon MAb (Clone 145-2C11) (25 UG)

货号： MAB484-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB484-SP
别名	CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
反应种属	Mouse
应用	Flow Cytometry,Immunoprecipitation,Cell depletion,CyTOF-reported,T Cell Stimulation
目标/特异性	Detects mouse CD3 epsilon. Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.¹ Binds to the CD3 epsilon -chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.^3,4
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Immunoprecipitation: Leo, O. et al. (1987) Proc. Natl. Acad. Sci. 84:1374. Cell depletion: Small, M. et al. (1994) J. Immunol. Meth. 167:103. T Cell Stimulation: 0.1-0.6 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	916 (Human); 12503 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Developmental heterogeneity in DNA packaging patterns influences T-cell activation and transmigration. Authors: Gupta S, Marcel N, Talwar S, Garg M, R I, Perumalsamy L, Sarin A, Shivashankar G PLoS ONE, 2012;7(9):e43718. Species: Mouse Sample Type: Whole Cells Application: Functional Assay Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation. Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA FASEB J., 2011;25(1):409-19. Species: Mouse Sample Type: Whole Cells Application: Bioassay Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells. Authors: Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA J. Immunol., 2010;184(4):2130-9. Species: Mouse Sample Type: Whole Cells Application: Neut Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking. Authors: Sinclair LV, Finlay D, Feijoo C, Cornish GH, Gray A, Ager A, Okkenhaug K, Hagenbeek TJ, Spits H, Cantrell DA Nat. Immunol., 2008;9(5):513-21. Species: Mouse Sample Type: Whole Cells Application: Functional Assay Protection of CD8+ T cells from activation-induced cell death by IL-18. Authors: Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H J. Leukoc. Biol., 2007;82(1):142-51. Species: Mouse Sample Type: Whole Cells Application: Functional Assay Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells. Authors: Bheeshmachar G, Purushotaman D, Sade H, Gunasekharan V, Rangarajan A, Sarin A J. Immunol., 2006;177(8):5041-50. Species: Mouse Sample Type: Whole Cells Application: Functional Assay Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay. Authors: Ogilvie RL, Abelson M, Hau HH, Vlasova I, Blackshear PJ, Bohjanen PR J. Immunol., 2005;174(2):953-61. Species: Mouse Sample Type: Whole Cells Application: Functional Assay Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration. Authors: Kipnis J, Cardon M, Avidan H, Lewitus GM, Mordechay S, Rolls A, Shani Y, Schwartz M J. Neurosci., 2004;24(27):6133-43. Species: Mouse Sample Type: Whole Cells Application: Functional Assay
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	H-2K^b-specific mouse cytotoxic T-lymphocyte
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CD3 is composed of five different polypeptides ranging from 16‑28 kDa that are associated with the T cell receptor (TCR) complex and serve as its signal transducing element. The CD3/TCR complex is expressed on T cells and thymocytes. Binding of immobilized anti-CD3 can cause T cell activation that leads to any of several consequences, depending on the conditions. Activation by anti-CD3 in the presence of IL 2 has been reported to induce cell death, apparently via apoptosis.⁴ The antibody can be used to induce cytolytic activity against non-specific targets and also to block TCR-mediated cytolytic killing.¹145-2C11 has been used in a variety of studies concerned with allograft rejection and graft-vs host reaction in mice.^{6, 7}
运输条件	Blue Ice
存放说明	4℃
参考文献	Leo, O. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1374. Portoles, P. et al. (1989) J. Immunol. 142:4169. Coulie, P.G. et al. (1991) Eur. J. Immunol. 21:1703. Ucker, D.S. J. Meyers and P.S. Obermiller. (1992) J. Immunol. 149:1583. Small, M. et al. (1994) J. Immunol. Meth. 167:103. Alegre, M.L. et al. (1991) J. Immunol. 146:1184. Hendrickson, M. et al. (1995) Transplantation 60:828.

参考图片

Mouse CD3 epsilon Antibody Stimulates Cell Proliferation in Mouse T Cells. Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) stimulates cell proliferation in mouse T cells in a dose-dependent manner, as measured by Resazurin (Catalog # AR002). The ED₅₀ for this effect is typically 0.1‑0.6 μg/mL.