| 货号 | MAB734-SP |
| 别名 | Hcrt 2; HCRT; Hcrt2; Hypocretin (orexin) neuropeptide precursor; Hypocretin 2; Hypocretin B; Hypocretin precursor; Hypocretin; Hypocretin2; Hypocretin-2; Orexin neuropeptide precursor; Orexin precursor; Orexin; OrexinB; PPORX; PPOX |
| 反应种属 | Human |
| 应用 | Immunohistochemistry |
| 目标/特异性 | Detects human Orexin B/Hypocretin‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Orexin A is observed. |
| 使用方法 | Immunohistochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3060 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | KLH-conjugated human Orexin B/Hypocretin‑2 synthetic peptide Accession # O43612 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Orexin A and Orexin B are neuropeptide hormones derived fromproteolytic processing of the Orexin precursor protein (Accession #O43612). Orexins are released from the hypothalamus and are involved inthe regulation of sleeping patterns, feeding, and metabolism. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Orexin B in Mouse Hypothalamus. Orexin B was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Mouse Anti-Human Orexin B Monoclonal Antibody (Catalog # MAB734) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the neuronal processes and synaptic boutons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem. Orexin B, p38 alpha, and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Mouse Anti-Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Anti-Mouse Integrin beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
