| 货号 | MAB1031-SP |
| 别名 | a disintegrin and metalloproteinase domain 8; ADAM 8; ADAM metallopeptidase domain 8; CD156a antigen; CD156a; CD156human leukocyte differentiation antigen; Cell surface antigen MS2; EC 3.4.24; EC 3.4.24.-; MGC134985; MS2; MS2disintegrin and metalloproteinase domain-containing protein 8 | 全称 | A Disintegrin and Metalloprotease-like Domain 8 |
| 反应种属 | Human |
| 应用 | Western Blot,Immunoprecipitation |
| 目标/特异性 | Detects human ADAM8 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with the ectodomains of recombinant human ADAM9, 10, 15, 17, and 19 is observed. Detects an epitope within aa 201‑497 of recombinant human ADAM8 which corresponds to the metalloprotease and disintegrin-like domains. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 101 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human ADAM8 ectodomain Asp158-Ser653 Accession # P78325 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | ADAM8, also known as cell surface antigen MS2 or CD156a, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). ADAM8 can cleave a variety of substrates and has been shown as a sheddase for the low affinity IgE receptor CD23 and the neural recognition molecule CHL1 (3, 4). Expression and regulation studies suggest that ADAM8 is a novel osteoclast stimulating factor and may play a role in asthma (5, 6). The 824 amino acid precursor of human ADAM8 consists of a signal peptide (residues 1 to 16), a pro peptide (residues 17 to 199), a metaloprotease domain (residues 200 to 400), a disintegrin-like domain (residues 408 to 494), a cysteine-rich region (residues 497 to 613), an EGF-like domain (residues 614 to 640), a transmembrane region (residues 656 to 676) and a cytoplasmic domain (residues 677 to 824). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human ADAM8 by Western Blot. Western blot shows Recombinant Human ADAM8 aa 158-497 (Catalog # 1031-AD). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ADAM8 Monoclonal Antibody (Catalog # MAB1031) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ADAM8 aa158-497 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
