| 货号 | MAB8531-SP |
| 别名 | BMP14; BMP-14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP-1; CDMP1LAP4; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; OS5; radotermin; SYNS2 | 全称 | Growth Differentiation Factor 5 |
| 反应种属 | Mouse |
| 应用 | Neutralization |
| 目标/特异性 | Detects mouse GDF-5/BMP-14 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) GDF-1, rmPro-GDF‑1, rmGDF-3, rmPro-GDF-3, rmGDF-6, rmGDF-7, rmGDF-8, rmGDF-9, rmPro-GDF-9, rmGDF-15, or recombinant human GDF-11 is observed. |
| 使用方法 | Neutralization: Measured by its ability to neutralize GDF‑5-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 3 µg/mL Recombinant Mouse GDF‑5. |
| 来源 | Monoclonal Rat IgG2B Clone # 143004 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8200 (Human); 14563 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse GDF-5/BMP-14 Ala376-Arg495 Accession # P43027 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1) and BMP-14, is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor beta (TGF‑ beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N‑terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80‑86% identity, define a subgroup within the BMP family. Like other TGF‑ beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Alkaline Phosphatase Production Induced by GDF‑5 and Neutralization by Mouse GDF‑5 Antibody. Recombinant Mouse GDF‑5 (Catalog # 853‑G5) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Mouse GDF‑5 (3 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GDF‑5 Monoclonal Antibody (Catalog # MAB8531). The ND50 is typically 2-10 µg/mL. |
