| 货号 | MAB582-SP |
| 别名 | Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18 | 全称 | Interleukin 22 |
| 反应种属 | Mouse |
| 应用 | Western Blot,CyTOF-ready,Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects mouse IL-22 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL-22 is observed, 15% cross-reactivity with recombinant human IL-22 is observed, and no cross-reactivity with recombinant mouse IL-10 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 50616 (Human); 50929 (Mouse); 500836 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-22 defines a novel immune pathway of antifungal resistance. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse IL-22 Leu34-Val179 Accession # Q9JJY9 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | IL-22 (Interleukin-22) is a cytokine that induces the production of reactive oxygen species, IL-6, IL-10, and TNF-alpha as well as neutrophil infiltration during inflammation. It also supports the integrity of epithelial barriers and induces epithelial cell proliferation during wound healing. IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is a shared component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29. IL-22 additionally binds to IL-22BP which blocks the interaction of IL-22 with IL-22 R. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of IL‑22 in EL‑4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse IL‑22 Monoclonal Antibody (Catalog # MAB582, dark orange histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab)2 Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. |
