| 货号 | MAB552-SP |
| 别名 | Hematopoietin-1; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha | 全称 | Interleukin 1 alpha |
| 反应种属 | Cotton Rat |
| 应用 | Western Blot,Neutralization |
| 目标/特异性 | Detects cotton rat IL‑1 alpha /IL‑1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant rat (rr) IL-1 alpha is observed and 20% cross-reactivity with recombinant mouse (rm) IL-1 alpha is observed. In Western blots, no cross-reactivity with recombinant human IL-1 alpha, rmIL-1 alpha, recombinant porcine IL-1 alpha, rrIL-1 beta, and rrIL-18 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 50 pg/mL Recombinant Cotton Rat IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A. |
| 来源 | Monoclonal Mouse IgG2A Clone # 140221 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant cotton rat IL‑1 alpha /IL‑1F1 Ser115-Pro269 Accession # AAK94011 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Cotton Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑1 alpha /IL‑1F1 and Neutralization by Cotton Rat IL‑1 alpha / IL‑1F1 Antibody. Recombinant Cotton Rat IL‑1 alpha /IL‑1F1 (Catalog # 552-R1) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL‑1 alpha /IL‑1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat IL‑1 alpha /IL‑1F1 Monoclonal Antibody (Catalog # MAB552). The ND50 is typically 1-5 µg/mL in the presence of concanavalin A (1.25 µg/mL). |
