| 货号 | MAB710-SP |
| 别名 | CD333; CEK; EC 2.7.10; fibroblast growth factor receptor 3; HSFGFR3EX; JTK4; JTK4thanatophoric dwarfism | 全称 | Fibroblast Growth Factor Receptor 3 |
| 反应种属 | Mouse |
| 应用 | Neutralization |
| 目标/特异性 | Detects mouse FGF R3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with the (IIIb) and (IIIc) isoforms of recombinant human FGF R3 is observed. Neutralizes the bioactivity of mouse FGF R3. Functions equally as well in the neutralization of recombinant mouse (rm) FGF R3 alpha (IIIb) and rmFGF R3 alpha (IIIc). |
| 使用方法 | Neutralization: Measured by its ability to neutralize FGF R3-mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 12 ng/mL Recombinant Mouse FGF R3 (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin. |
| 来源 | Monoclonal Rat IgG2A Clone # 136812 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2261 (Human); 14184 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Prox1 promotes lineage-specific expression of fibroblast growth factor (FGF) receptor-3 in lymphatic endothelium: a role for FGF signaling in lymphangiogenesis. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse FGF R3 Glu21-Tyr369 (Asn310Ile, Ala354 del) (predicted) Accession # NP_001156689 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1‑4, are known. All four genes for FGF Rs encode proteins with an N‑terminal signal peptide, three immunoglobulin (Ig)‑like domains, an acid‑box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine‑kinase domain. Multiple forms of FGF R1‑3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform. Only the alpha isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1‑3, involving the C‑terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N‑terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1‑3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are being investigated. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
FGF R3 Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Mouse FGF R3 Antibody. Recombinant Mouse FGF R3 (IIIc) Fc Chimera (Catalog # 710-MF) inhibits Recombinant Human FGF acidic (Catalog # 232‑FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Mouse FGF R3 (IIIc) Fc Chimera (12 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse FGF R3 Monoclonal Antibody (Catalog # MAB710). The ND50 is typically 3-12 µg/mL in the presence of heparin (10 µg/mL). |
